Availability
Please contact us to check the availability
Specificity
Binding to Biotin and Biotin conjugates
Species reactivity
not applicable
Please contact us to check the availability
Binding to Biotin and Biotin conjugates
not applicable
Conjugate in buffer containing 10 mM HEPES, 0.15 M NaCl, 0.08% NaN3, 1 mM Mg2+, 0.1 mM Zn2+, pH7.5
Enzyme Conjugates
Streptomyces avidinii
none
none
liquid concentrate
dilute 1:100 - 1:1000
1 mg/ml
none
none
IHC, ELISA , WB
Suitable substrates for alkaline phosphatase reaction in immunohistochemistry are e.g. Vector Red (Art. No. SK-5100), VECTOR Blue (Art. No. SK-5300) or Fast Red TR. In immuno blots BCIP/NBT (Art. No. SK-5400) or chemiluminescence substrate DuoLUX (Art. No. SK-6605) may be used. For the substrate reaction an alkaline TRIS buffer is recommended (Art. Nr. PU004). Please observe the instructions of the manufacturer.
Store at 2-8°C
Go to UniProt/SwissProt
Go to PubMed
Gentaur products are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
