Category
Antibody
Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Form
Antigen affinity purified
Antibody
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Antigen affinity purified
Unconjugated
Polyclonal antibody
Alkaline Phosphatase (tissue-nonspecific)
Rabbit (Oryctolagus cuniculus)
Polyclonal (rabbit origin)
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
WB, IHC-P
Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
Titration of the Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
This Alkaline Phosphatase antibodyis to be used only for research purposes and not for diagnostics..
P05186
Antigen affinity
Alkaline phosphatase, tissue-nonspecific isozyme is an enzyme that in humans is encoded by the ALPL gene. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. Liver/bone/kidney alkaline phosphatase, known as tissue-nonspecific, acts physiologically as a lipid-anchored phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP) ectophosphatase. Mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects.
Amino acids 21-35 (EKEKDPKYWRDQAQE) of human ALPL were used as the immunogen for this tissue-nonspecific Alkaline Phosphatase antibody.
The lyophilized Alkaline Phosphatase antibody can be stored at 4oC to -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. After reconstitution, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
tissue
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
6
anticorps