Category
Antibody
Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
Form
Antigen affinity purified
Antibody
In 1X PBS, pH 7.4, with 0.09% sodium azide
Antigen affinity purified
Unconjugated
Polyclonal antibody
Placental Alkaline Phosphatase
Rabbit (Oryctolagus cuniculus)
Polyclonal (rabbit origin)
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
WB, IHC, ELISA
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50
Titration of the placental Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
This placental Alkaline Phosphatase antibodyis to be used only for research purposes and not for diagnostics..
P05187
Antigen affinity
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.
A portion of amino acids 56-83 from the human protein was used as the immunogen for this placental Alkaline Phosphatase antibody.
Aliquot the placental Alkaline Phosphatase antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
anticorps
