Category
Antibody
Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
Form
Antigen affinity purified
Antibody
In 1X PBS, pH 7.4, with 0.09% sodium azide
Antigen affinity purified
Unconjugated
Polyclonal antibody
Anti-Alkaline Phosphatase
Rabbit (Oryctolagus cuniculus)
Polyclonal (rabbit origin)
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
WB, IHC, IF, ELISA
Western blot: 1:1000,IHC (Paraffin): 1:50-100,Immunofluorescence: 1:100
Titration of the anti-Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
This anti-Alkaline Phosphatase antibodyis to be used only for research purposes and not for diagnostics..
P09923
Antigen affinity
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.
A portion of amino acids 274-304 from human ALPI was used as the immunogen for this anti-Alkaline Phosphatase antibody.
Aliquot the anti-Alkaline Phosphatase antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
anticorps
