Category
Antibody
Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
Form
Purified
Antibody
In 1X PBS, pH 7.4, with 0.09% sodium azide
Purified
Unconjugated
Polyclonal antibody
Alkaline Phosphatase
Rabbit (Oryctolagus cuniculus)
Polyclonal (rabbit origin)
Human (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
FACS, IHC, WB, IF, ELISA
Western blot: 1:1000,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50,IHC (Paraffin): 1:10-1:50
Titration of the Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
This Alkaline Phosphatase antibodyis to be used only for research purposes and not for diagnostics..
P05186
Purified
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The genes for the first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. This protein is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms.
A portion of amino acids 217-246 from human ALPL was used as the immunogen for this Alkaline Phosphatase antibody.
Aliquot the Alkaline Phosphatase antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
anticorps
