Alkaline Phosphatase Antibody

Size

0.4 ml

Catalog No

F44129-0.4ML

Price

406€

Category

Antibody

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Form

Purified

Alkaline Phosphatase Antibody

Conjugation

Unconjugated

Clone

Polyclonal antibody

Recognised antigen

Alkaline Phosphatase

Alkaline Phosphatase Antibody

Host animal

Rabbit (Oryctolagus cuniculus)

Clonality

Polyclonal (rabbit origin)

Species reactivity

Human (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

Alkaline Phosphatase Antibody

Tested applications

FACS, IHC, WB, IF, ELISA

Recommended dilutions

Western blot: 1:1000,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50,IHC (Paraffin): 1:10-1:50

Notes

Titration of the Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Alkaline Phosphatase Antibody

Intented use

This Alkaline Phosphatase antibodyis to be used only for research purposes and not for diagnostics..

Uniprot #

P05186

Purity

Purified

Alkaline Phosphatase Antibody

Description

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The genes for the first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. This protein is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms.

Immunogen

A portion of amino acids 217-246 from human ALPL was used as the immunogen for this Alkaline Phosphatase antibody.

Storage

Aliquot the Alkaline Phosphatase antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.

Alkaline Phosphatase Antibody

Properties

If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

French translation

anticorps

Alkaline Phosphatase Antibody