Category
Antibody
Concentration
0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Form
Purified
Antibody
0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Purified
Unconjugated
Polyclonal antibody
Acid Phosphatase / ACP1
Rabbit (Oryctolagus cuniculus)
Polyclonal (rabbit origin)
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
WB
Western blot: 1-3ug/ml
Provided assay concentrations are suggestions only, ACP1 antibody titration may be required for optimal results. 1. This antibody will detect the A, C and D isoforms of Acid Phosphatase.
1X Phosphate Buffered Saline (PBS), pH 7.4
This Acid Phosphatase / ACP1 antibodyis to be used only for research purposes and not for diagnostics..
52
Purified by protein A Affinity Chromatography
Acid phosphatases (AP) are a broad family of enzymes that differ widely in their AA length and homology, molecular weight and tissue of origin. They function to remove phosphate groups (or dephosphorylate) from proteins and perform best under acidic conditions. Disease states are sometimes characterized by abnormally high or low levels of Acid phosphatase, leading to them being used as serological and histological disease markers. Prostatic Acid Phosphatase (PAP) was initially used as a prostate cancer marker but was later replaced with Prostate Serum Antigen (PSA), a more sensitive early stage cancer marker.
A recombinant protein fragment from the N-terminal portion of Acid Phosphatase / ACP1 was used as the immunogen for this antibody. This AA sequence is common to the A, C and D isoforms.
Aliquot the ACP1 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
anticorps
