Category
Antibody
Concentration
0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Form
Purified
Antibody
0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Purified
Unconjugated
Polyclonal antibody
Acid Phosphatase (Isoform 2 specific)
Rabbit (Oryctolagus cuniculus)
Polyclonal (rabbit origin)
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
WB
Western blot: 1-3ug/ml
Provided assay concentrations are suggestions only, Acid Phosphatase antibody titration may be required for optimal results. 1. This antibody is specific to isoforms 2 (or B) and E of Acid Phosphatase.
1X Phosphate Buffered Saline (PBS), pH 7.4
This Acid Phosphatase antibodyis to be used only for research purposes and not for diagnostics..
52
Purified by protein A Affinity Chromatography
Acid phosphatases are a broad family of enzymes that differ widely in their amino acid length and homology, molecular weight and tissue of origin. They function to remove phosphate groups (or dephosphorylate) from proteins and perform best under acidic conditions. Disease states are sometimes characterized by abnormally high or low levels of Acid Phosphatase, leading to them being used as serological and histological disease markers. Prostatic Acid Phosphatase (PAP) was initially used as a prostate cancer marker but was later replaced with Prostate Serum Antigen (PSA), a more sensitive early stage cancer marker.
The amino acid sequence used as immunogen for this antibody is specific to isoforms 2 (or B) and E of Acid Phosphatase.
Aliquot the Acid Phosphatase antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
anticorps
