Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog; PTEN; MMAC1; TEP1
Synthetic phosphopeptide derived from human PTEN around the phosphorylation site of Serine 380 and 382 and Threonine 383.
P60484
Human,Mouse,Rat
WB IHC
WB: 1:500~1:1000 IHC: 1:50~1:200
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
p-PTEN (S380/T382/T383) polyclonal antibody detects endogenous levels of PTEN protein only when phosphorylated at Ser380/T382/T383.
Western blot (WB) analysis of PTEN (p-S380/T382/T383) polyclonal antibody at 1:1000 dillution Lane1:Hela whole cell lysate(30μg) Lane2:Hela treated with PMA(60ng/ml, 30min) whole cell lysate(30μg) Lane3:H9C2 whole cell lysate(30μg) Lane4:H9C2 treated with PMA(60ng/ml, 30min) whole cell lysate(30μg)
~ 47, 54 kDa
For research use only, not for use in diagnostic procedure.
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
anticorps
