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Lyophilized
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-PPase,UniProt P31414, TAIR AT1G15690
Raised in
Rabbit
Lyophilized
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-PPase,UniProt P31414, TAIR AT1G15690
Rabbit
Polyclonal
Polyclonal
Serum
See included datasheet or contact our support service
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
western blot (WB)
collection of antibodies to tonoplast proteins AS09 492 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)AS09 493 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)AS09 494 | anti-TIP2;1 (delta) | Delta-VM23AS09 510 | anti-TIP2;1 | tonoplast intrinistic protein 2-1AS09 510 | anti-TIP2;2 | tonoplast intrinistic protein 2-2AS09 511 | anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal)Plant protein extraction bufferSecondary antibodies
1: 8000 (ELISA), 1: 2000 with standard ECL (WB), 1: 100 (IL)
80.8 | 73 kDa (Arabidopsis thaliana)
Arabidopsis thaliana, Cucumis sativus, Dunaliella bardawil, Hordeum vulgare, Nicotiana tabacum, Picea abies
dicots including: Ricinus communis, Vitis vinifera, Saccharum officinarum, monocots including: Oryza sativa, Zea mays, Vigna radiata, trees: Populus trichocarpa
no confirmed exceptions from predicted reactivity known in the moment
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Migocka et al. (2015). Cucumber Metal Transport Protein CsMTP9 is a plasma membrane H+ -coupled antiporter involved in the Mn2+ and Cd2+ efflux from root cells. Plant J. 2015 Oct 20. doi: 10.1111/tpj.13056. Migocka et al. (2014). Molecular and biochemical properties of two P 1B2 -ATPases, CsHMA3 and CsHMA4, from cucumber. Plant Cell Environ. 2014 Sep 11. doi: 10.1111/pce.12447.
V-PPase (pyrophosphate-energized vacuolar membrane proton pump 1), EC=3.6.1.1, acts to establish proton gradient of often higher magnitude than H+-ATPase. Is involved in auxin transport and NaCl and drought tolerance. Alternative names: pyrophosphate-energized inorganic pyrophosphatase 1, H(+)-PPase 1, vacuolar proton pyrophosphatase 1, vacuolar proton pyrophosphatase 3
Antibodies will detect target protein in 1 µg of a crude membrane preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, less than µg load per well should be sufficient.Protocol of isolation of vacuolar membrane is available here. Please note that vacuolar membrane accounts for 10 % of protein of the total memrbanes of growing plant tissues. Recovery of vacuolar membrane following this protocol will depend upon amount of starting material used.
P31414
AT1G15690
