Available ordering format
Lyophilized in phosphate buffer saline, labelled with biotin
Immunogen
native acid phosphatase purified from Solanum tuberosum
Raised in
Rabbit
Lyophilized in phosphate buffer saline, labelled with biotin
native acid phosphatase purified from Solanum tuberosum
Rabbit
Polyclonal
Polyclonal
Purified IgG
For reconstitution please add 1 ml of sterile destilled water.
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
AS09 520 | anti-APase | acid phospgatase rabbit anitbodyPlant protein extraction bufferSecondary antibodies
1: 1000 to 1: 4000 for techniques listed above
37.4 kDa
Solanum tuberosum
Glycine max, Ricinus communis
no confirmed exceptions from predicted reactivity known in the moment
antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
not available at the moment
Acid phosphatase is an enzyme which functions in hydrolysis of phosphate esters.
biotin/IgG protein molar ratio (B/P) is approximately 6.6. No foreign proteins are added. Marker used for lebling is N-hydroxysuccinimidobiotin.
Refer to NCBI
Refer to NCBI
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Biotinylated
