Size

100 μg

Catalog No

X2737P

Price

420€

Buy: Rabbit anti PH domain leucine-rich repeat-containing protein phosphatase 2

Long description

PHLPP2 is a protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor.

Antibody come from

Synthetic peptide derived from human DICER1 protein.

Rabbit anti PH domain leucine-rich repeat-containing protein phosphatase 2

Other description

Provided as solution in phosphate buffered saline with 0.08% sodium azide. Antigen Immunoaffinity Purification

Clone

Polyclonal

Antigen-antibody binding interaction

Rabbit anti PH domain leucine-rich repeat-containing protein phosphatase 2 Antibody

Antibody is raised in

Rabbit

Antibody's reacts with

Human

Antibody's reacts with these species

This antibody doesn't cross react with other species

Antibody's specificity

No Data Available

Application

Immunohistochemistry, Western Blot, ELISA

Antibody's suited for

Antibody can be used for immunohistochemical staining (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Storage

-20ºC

Relevant references

1. Zhang, H., et al. ÔSingle processing center models for human Dicer and bacterial RNase III.Õ Cell, 118, 57-68 (2004)_x000B__x000B_2. Meister, G., et al. ÔIdentification of novel argonaute-associated proteins.Õ Curr. Biol., 15, 2149-2155 (2005)_x000B__x000B_3. Haase, A.D., et al. ÔTRBP, a regu1ator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing.Õ EMBO Rep., 6, 961-967 (2005)_x000B__x000B_4. Kok, K.H., et al. ÔHuman TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA.Õ J. Biol. Chem., 282, 17649-17657 (2007)_x000B__x000B_5. Melo, S.A., et al. ÔA TARBP2 mutation in human cancer impairs microRNA processing and DICER1 function.Õ Nat. Genet., 41, 365-370 (2009)

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Latin name

Oryctolagus cuniculus

Rabbit anti PH domain leucine-rich repeat-containing protein phosphatase 2