Size

50 μg

Catalog No

X1645B

Price

168€

Buy: Sphingosine 1-Phosphate Phosphatase 2 - Blocking Peptide

Long description

Sphingosine 1-phosphate (S1P) is a highly bioactive lipid that has a myriad of biological effects both intracellularly as a second messenger and extracellularly by binding to the S1P(1-5)/G-protein-coupled receptors of the endothelial differentiation gene family. Intracellularly, at least two enzymes, sphingosine kinase (1 and 2) and S1P phosphatase, regulate the activity of S1P by governing the phosphorylation of S1P. SPPase2 is localized to the endoplasmic reticulum. The enzymatic activity and localization of SPPase2 are similar to SPPase1. The tissue expression of SPPase2 was different from that of SPPase1. SPPase2 is an important member of the SPPase family which play a role in attenuating intracellular S1P signaling.

Antibody come from

Recombinant enyzme produced in E. coli

Sphingosine 1-Phosphate Phosphatase 2 - Blocking Peptide

Other description

Provided in 25 mM Tris-HCl, 75 mM NaCl, pH 8.0, 0.05% Tween, 5 mM DTT and 50% glycerol.

Clone

not specified

Antigen-antibody binding interaction

Sphingosine 1-Phosphate Phosphatase 2 - Blocking Peptide

Antibody is raised in

see techfile

Antibody's reacts with

Human

Antibody's reacts with these species

This antibody doesn't cross react with other species

Antibody's specificity

No Data Available

Application

Antibody function blocking

Antibody's suited for

3.8 nmole/min/µg of enzyme; Detemined using pNPP; Reaction conditions: 50 µM pNPP, 10 min incubation at 30¼C, 0.5 µg enzyme.

Storage

-20ºC

Relevant references

[1] Kloeker S., Wadzinski B.E.; Purification and identification of a novel subunit of protein serine/threonine phosphatase 4.; J. Biol. Chem. 274:5339-5347(1999)._x000B_[2] Wada T., Miyata T., Inagi R., Nangaku M., Wagatsuma M., Suzuki D., Wadzinski B.E., Okubo K., Kurokawa K.; Cloning and characterization of a novel subunit of protein serine/threonine phosphatase 4 from mesangial cells.; J. Am. Soc. Nephrol. 12:2601-2608(2001)._x000B_[3] Zhang X, Ozawa Y, Lee H, Wen YD, Tan TH, Wadzinski BE, Seto E. Histone deacetylase 3 (HDAC3) activity is regulated by interaction with protein serine/threonine phosphatase 4._x000B_Genes Dev. 2005 Apr 1;19(7):827-39. _x000B_[4] Kloeker S, Wadzinski BE. Purification and identification of a novel subunit of protein serine/threonine phosphatase 4. J Biol Chem. 1999 Feb 26;274(9):5339-47. _x000B__x000B_

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Test

You can block the antibody by the specific target amino acid sequence of peptide.

Properties

blocking peptide

Description

Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.