Rabbit anti Sphingosine 1-Phosphate Phosphatase 2

Size

10MinibLots

Catalog No

X1637P

Price

436€

Category

Primary Antibodies

Long description

Sphingosine 1-phosphate (S1P) is a highly bioactive lipid that has a myriad of biological effects both intracellularly as a second messenger and extracellularly by binding to the S1P(1-5)/G-protein-coupled receptors of the endothelial differentiation gene family. Intracellularly, at least two enzymes, sphingosine kinase (1 and 2) and S1P phosphatase, regulate the activity of S1P by governing the phosphorylation of S1P. SPPase2 is localized to the endoplasmic reticulum. The enzymatic activity and localization of SPPase2 are similar to SPPase1. The tissue expression of SPPase2 was different from that of SPPase1. SPPase2 is an important member of the SPPase family which play a role in attenuating intracellular S1P signaling.

Antibody come from

Recombinant enyzme produced in E. coli

Rabbit anti Sphingosine 1-Phosphate Phosphatase 2

Other description

Provided in 25 mM Tris-HCl, 75 mM NaCl, pH 8.0, 0.05% Tween, 5 mM DTT and 50% glycerol. Antigen Immunoaffiinity Purification

Clone

Polyclonal

Antigen-antibody binding interaction

Rabbit anti Sphingosine 1-Phosphate Phosphatase 2 Antibody

Rabbit anti Sphingosine 1-Phosphate Phosphatase 2

Antibody is raised in

Rabbit

Antibody's reacts with

Human

Antibody's reacts with these species

This antibody doesn't cross react with other species

Rabbit anti Sphingosine 1-Phosphate Phosphatase 2

Antibody's specificity

No Data Available

Application

Western Blot

Antibody's suited for

3.3 pmole/min/µg of enzyme; Detemined using DiFMUP; Reaction conditions: 100 µM DiFMUP, 10 min incubation at 30¼C, 5 µg enzyme.

Rabbit anti Sphingosine 1-Phosphate Phosphatase 2

Storage

-80ºC

Relevant references

[1] Cates C.A., Michael R.L., Stayrook K.R., Harvey K.A., Burke Y.D., Randall S.K., Crowell P.L., Crowell D.N.; Prenylation of oncogenic human PTP(CAAX) protein tyrosine phosphatases.; Cancer Lett. 110:49-55(1996)._x000B_[2] Peng Y., Genin A., Spinner N.B., Diamond R.H., Taub R.; The gene encoding human nuclear protein tyrosine phosphatase, PRL-1. Cloning, chromosomal localization, and identification of an intron enhancer.; J. Biol. Chem. 273:17286-17295(1998)._x000B_[3] Gjorloff-Wingren A, Saxena M, Han S, Wang X, Alonso A, Renedo M, Oh P, Williams S, Schnitzer J, Mustelin T. Subcellular localization of intracellular protein tyrosine phosphatases in T cells._x000B_Eur J Immunol. 2000 Aug;30(8):2412-21. _x000B_[4] Yarovinsky TO, Rickman DW, Diamond RH, Taub R, Hageman GS, Bowes Rickman C._x000B_Expression of the protein tyrosine phosphatase, phosphatase of regenerating liver 1, in the outer segments of primate cone photoreceptors. Brain Res Mol Brain Res. 2000 Apr 14;77(1):95-103. _x000B_[5] Carter DA. Expression of a novel rat protein tyrosine phosphatase gene. Biochim Biophys Acta. 1998 Nov 8;1442(2-3):405-8. _x000B_[6] Zeng Q, Hong W, Tan YH. Mouse PRL-2 and PRL-3, two potentially prenylated protein tyrosine phosphatases homologous to PRL-1. Biochem Biophys Res Commun. 1998 Mar 17;244(2):421-7. _x000B_[7] Cates CA, Michael RL, Stayrook KR, Harvey KA, Burke YD, Randall SK, Crowell PL, Crowell DN Prenylation of oncogenic human PTP(CAAX) protein tyrosine phosphatases. Cancer Lett. 1996 Dec 20;110(1-2):49-55. _x000B_[8] Takano S, Fukuyama H, Fukumoto M, Kimura J, Xue JH, Ohashi H, Fujita J. PRL-1, a protein tyrosine phosphatase, is expressed in neurons and oligodendrocytes in the brain and induced in the cerebral cortex following transient forebrain ischemia. Brain Res Mol Brain Res. 1996 Aug;40(1):105-15. _x000B_

Protein number

see ncbi

Rabbit anti Sphingosine 1-Phosphate Phosphatase 2

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Rabbit anti Sphingosine 1-Phosphate Phosphatase 2

Latin name

Oryctolagus cuniculus

Rabbit anti Sphingosine 1-Phosphate Phosphatase 2