Size

100 μg

Catalog No

X1233M

Price

304€

Buy: Mouse anti Placental Alakaline Phosphatase (PLAP)

Long description

Placental alkaline phosphatase is a throphoblast-derived antigen from human placenta. It is a membrane-associated antigen present in high frequency in gynacological cancers and in lower frequency in other tumors. This antibody can be used as an epitope-defined marker in germ cell tumors and is applicable for immunosorbent purification, identification of hidden determinants on the native enzyme and for radioimmuno-localization.

Antibody come from

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with purified placental alkaline phosphatase and mouse myeloma cells

Mouse anti Placental Alakaline Phosphatase (PLAP)

Other description

Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography

Clone

H7E8

Antigen-antibody binding interaction

Mouse anti Placental Alakaline Phosphatase (PLAP) Antibody

Antibody is raised in

Mouse

Antibody's reacts with

Human

Antibody's reacts with these species

This antibody doesn't cross react with other species

Antibody's specificity

No Data Available

Application

ELISA, Western Blot, Immunohistochemistry (Frozen Sections)

Antibody's suited for

This antibody can be used for Western blotting, ELISA and immunohistochemistry on frozen tissue sections. Optimal concentration should be evaluated by serial dilutions.

Storage

-20ºC

Relevant references

1. Millan, J., et al. 'Antigenic determinants of human placental and testicular alkaline phosphatase as mapped by monoclonal antibodies.' Eur. J. Biochem. 1983, 136, 1-12._x000B__x000B_2. Skynner, M.J. et al., 'Transgenic mice ubiquitously expressing human placental alkaline phosphatase (PLAP): an additional reporter gene for use in tandem with beta-galactosidase (lacZ).' Int. J. Dev. Biol. 1999, 43, 85-90._x000B__x000B_3. Koshida, K., et al. 'A potential use of a monoclonal antibody to placental alkaline phosphatase (PLAP) to detect lymph node metastases of seminoma.' J. Urol. 1996, 155, 337-341.

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus

Mouse anti Placental Alakaline Phosphatase (PLAP)