Sheep anti Protein Phosphatase 4 R1 (X/A)

Size

100 μg

Catalog No

P210P

Price

304€

Category

Primary Antibodies

Long description

Protein serine/threonine phosphatase 4R1 is a protein which is associated with the catalytic subunit of protein serine/threonine phosphatase 4 (PP4C) and may possibly regulate the activity of the enzyme. PP4C is 65% identical to PP2AC at the amino acid level and has been placed in the type 2A family of phosphatases. PP4C has been highly conserved between species sharing 91% amino acid identity between human and Drosophila PP4C is predominantly localized in the nucleus in rat brain and liver but is most highly expressed in testis. Additionally, PP4C was demonstrated to be an essential enzyme in the development of Drosophila embryos. The expression of PP4C was reduced to 25% of the normal protein level in a mutant strain of Drosophila termed centrosomes minus microtubules (cmm). An interesting characteristic of the cmm phenotype is the presence of regions of cells that are unable to complete mitosis because no microtubules exist to connect chromatin and centrosomes. This phenotype implicates PP4C in the regulation of the nucleation and/or stabilization of microtubules. These data taken together indicate that PP4 has a crucial cellular function, although a physiological substrate for PP4 has not yet been identified.

Antibody come from

Antibody raised against synthetic peptide corresponding to amino acids 38 to 52 of the human protein phosphatase 4R1.

Sheep anti Protein Phosphatase 4 R1 (X/A)

Other description

Provided as solution in phosphate buffered saline with 0.08% sodium azide. Ammonium Sulfate Precipitation

Clone

Polyclonal

Antigen-antibody binding interaction

Sheep anti Protein Phosphatase 4 R1 (X/A) Antibody

Sheep anti Protein Phosphatase 4 R1 (X/A)

Antibody is raised in

Sheep

Antibody's reacts with

Human, Mouse, Rat

Antibody's reacts with these species

This antibody doesn't cross react with other species

Sheep anti Protein Phosphatase 4 R1 (X/A)

Antibody's specificity

No Data Available

Application

Western Blot

Antibody's suited for

Detects protein phosphatase X/A (PP4 R1) protein by Western blot (5-10 µg/ml). Detects a 125 kDa band in bovine testes lysate. Optimal concentration should be evaluated by serial dilutions.

Sheep anti Protein Phosphatase 4 R1 (X/A)

Storage

-20ºC

Relevant references

Kloeker, S., et al. Purification and identification of a novel subunit of protein serine/threonine phosphatase 4. J. Biol. Chem. 1999, 274, 5339-5347.

Protein number

see ncbi

Sheep anti Protein Phosphatase 4 R1 (X/A)

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Sheep anti Protein Phosphatase 4 R1 (X/A)