Size

1 mg

Catalog No

NE124/Pab

Price

400€

Buy: Rabbit anti Escherichia coli Phosphatase alkaline

Long description

The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own choice.

Antibody come from

Alkaline phosphatase isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure

Rabbit anti Escherichia coli Phosphatase alkaline

Other description

Purified hyperimmune rabbit IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran. No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Clone

Polyclonal

Antigen-antibody binding interaction

Rabbit anti Escherichia coli Phosphatase alkaline Antibody

Antibody is raised in

Rabbit

Antibody's reacts with

Bacteria

Antibody's reacts with these species

Cross-reactivities anti enzymes of other sources may occur but have not been determined

Antibody's specificity

Affinity purified antibodies of polyclonal Rabbit antiSerum to alkaline phosphatase from Escherichia coli

Research interest

Enzymes, Microbiology

Application

Immunoprecipitation

Antibody's suited for

Immunoprecipitation. Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between10,000 and 1/400,000.

Storage

The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days.

Relevant references

no information yet

Protein number

P00634

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Latin name

Oryctolagus cuniculus

Rabbit anti Escherichia coli Phosphatase alkaline