Recombinant Human Alkaline Phosphatase/ALPL (C-6His)

Size

1 mg

Catalog No

C554-1000

Price

2283€

Description

Recombinant Human Alkaline Phosphatase is produced by our Mammalian expression system and the target gene encoding Leu18-Ser502 is expressed with a 6His tag at the C-terminus.

Species reactivity

Human

Origin

Human Cells

Recombinant Human Alkaline Phosphatase/ALPL (C-6His)

Peptide sequence

LVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSVDHHHHHH

Estimated molecular weight

54,5 kDa

Protein purity

Greater than 95% as determined by reducing SDS-PAGE.

Recombinant Human Alkaline Phosphatase/ALPL (C-6His)

Endotoxin level

Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

Shipping condition

Dry Ice/ice packs

Package form

Supplied as a 0.2 µm filtered solution of 20mM HEPES, 150mM NaCl, 2mM MgSO4, 0.1mM ZnCl2, pH 7.5.

Recombinant Human Alkaline Phosphatase/ALPL (C-6His)

Storage conditions

Store at below -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.

Reconstitution conditions

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

UniProt number

P05186

Recombinant Human Alkaline Phosphatase/ALPL (C-6His)

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Source

Recombinants or rec. proteins

Group

recombinants

Recombinant Human Alkaline Phosphatase/ALPL (C-6His)
Recombinant Human Alkaline Phosphatase/ALPL (C-6His)