anti- PTEN antibody

Size

2X100μg

Catalog No

FNab09821

Price

320€

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

anti- PTEN antibody

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)

Background

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.

Immunogen

phosphatase and tensin homolog

anti- PTEN antibody

Synonyms

MMAC1, TEP1

Calculated MW

55 kDa

Uniprot ID

P60484

anti- PTEN antibody

Specificity

Human, Mouse, Rat, Monkey

Tested Application

ELISA, WB, IHC

Recommended dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:100

anti- PTEN antibody

Image1

Immunohistochemistry of paraffin-embedded human breast hyperplasia tissue slide using FNab09821( PTEN Antibody) at dilution of 1:600

Image4

fetal human brain tissue were subjected to SDS PAGE followed by western blot with FNab09821( PTEN Antibody) at dilution of 1:1000

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

anti- PTEN antibody

Properties

If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

French translation

anticorps

anti- PTEN antibody