Human ALPI(Alkaline Phosphatase, Intestinal) ELISA Kit

Size

96T

Catalog No

ELK1758

Price

608€

Alternative Names

CIP; IAP; Calf Intestinal Alkaline Phosphatase

Assay Type

Sandwich

Sensitivity

0.55ng/mL

Human ALPI(Alkaline Phosphatase, Intestinal) ELISA Kit

Standard

100ng/mL

Detection range

1.56-100ng/mL

Assay length

3h

Human ALPI(Alkaline Phosphatase, Intestinal) ELISA Kit

Research Area

Enzyme & Kinase;Metabolic pathway;Gastroenterology;

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, Intestinal (ALPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase, Intestinal (ALPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase, Intestinal (ALPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alkaline Phosphatase, Intestinal (ALPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Human ALPI(Alkaline Phosphatase, Intestinal) ELISA Kit

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Human ALPI(Alkaline Phosphatase, Intestinal) ELISA Kit