Human ACP5(Acid Phosphatase 5, Tartrate Resistant) ELISA Kit

Alternative Names

TRAP; TrATPase; Tartrate Resistant Acid Phosphatase; Type 5 acid phosphatase

Assay Type

Sandwich

Sensitivity

0.129ng/mL

Human ACP5(Acid Phosphatase 5, Tartrate Resistant) ELISA Kit

Standard

20ng/mL

Detection range

0.312-20ng/mL

Assay length

3h

Human ACP5(Acid Phosphatase 5, Tartrate Resistant) ELISA Kit

Research Area

Enzyme & Kinase;Metabolic pathway;Hepatology;

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acid Phosphatase 5, Tartrate Resistant (ACP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acid Phosphatase 5, Tartrate Resistant (ACP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acid Phosphatase 5, Tartrate Resistant (ACP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acid Phosphatase 5, Tartrate Resistant (ACP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Human ACP5(Acid Phosphatase 5, Tartrate Resistant) ELISA Kit

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Human ACP5(Acid Phosphatase 5, Tartrate Resistant) ELISA Kit