Immunogen
Synthesized peptide derived from Internal of human vimentin.
Antibody's target
Human Vimentin
Antibody's full description
Rabbit Anti-Human Vimentin Polyclonal
100 µl
SPC-1338D-ALP
350€
Synthesized peptide derived from Internal of human vimentin.
Human Vimentin
Rabbit Anti-Human Vimentin Polyclonal
Neuroscience, Cell Structure, Cell Signaling, Neurodegeneration, Cancer
Polyclonal Antibodies
CTRCT30 Antibody, Epididymis luminal protein 113 Antibody, HEL113 Antibody, VIM Antibody, VIME_HUMAN Antibody, Vimentin Antibody
WB, IHC, ELISA
Rabbit
Human, Mouse, Rat
WB (1:1000), IHC (1:100), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~53kDa, endogenous levels of total vimentin protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1338 was sufficient for detection of Vimentin in 10 µg of HepG2 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cytoplasm
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Vimentin is a type III intermediate filament (IF) protein. All IF proteins are highly expressed and vimentin is the major cytoskeletal component of mesechymal, and therefore Vimentin is used as a marker of mesenchymally-derived cells or cells undergoing EMT during both normal development and metastic progression. Functionally it also serves a significant role in supporting and anchording the organelles in the cytosol. Clinical significance includes being used as a sarcoma tumor marker, and it has also been used in the diagnosis of rheumatoid arthritis.
1. Katsumoto T., Mitsushima A., Kurimura T. (1990). Biol Cell. 68(2): 139–146. 2. Leader M, Collins M, Patel J, Henry K (1987). Histopathology 11(1): 63–72. 3. "Immunohistochemistry from the Washington Animal Disease Diagnostic laboratory (WADDL)of the College of Veterinary Medicine, Washington State University". Retrieved 2009-03-14.
6/Sep/2016
NP_003371.2
7431
P08670
Refer to PubMed
To be tested
Immunohistochemistry analysis using Rabbit Anti-Vimentin Polyclonal Antibody (SPC-1338). Tissue: Colon Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Vimentin Polyclonal Antibody (SPC-1338) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human HepG2 cell lysates showing detection of ~54kDa Vimentin protein using Rabbit Anti-Vimentin Polyclonal Antibody (SPC-1338). Lane 1: Human HepG2 cells, treated with Adriamycin (0.5uM, 5hours). Lane 2: Untreated. Primary Antibody: Rabbit Anti-Vimentin Polyclonal Antibody (SPC-1338) at 1:1000. Predicted/Observed Size: ~54kDa. Rabbit Anti-Vimentin Antibody used in Immunohistochemistry (IHC) on Colon Carcinoma Tissue (SPC-1338) | Rabbit Anti-Vimentin Antibody used in Western blot (WB) on HepG2 cell lysates (SPC-1338)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
