Immunogen
Peptide sequence around aa.18~22(S-N-S-E-E) derived from Human Twist1
Antibody's target
Human Twist
Antibody's full description
Rabbit Anti-Human Twist Polyclonal
100 µl
SPC-1336D-ALP
350€
Peptide sequence around aa.18~22(S-N-S-E-E) derived from Human Twist1
Human Twist
Rabbit Anti-Human Twist Polyclonal
Neuroscience, Cell Signaling, Epigenetics, Cancer, Cardiovascular System, Heart
Polyclonal Antibodies
ACS3 Antibody, bHLHa38 Antibody, SCS Antibody, TWIST Antibody, Twist basic helix loop helix transcription factor 1 Antibody, Twist homolog 1 Antibody, Twist related protein 1 Antibody, TWIST1 Antibody
WB, IHC
Rabbit
Human, Mouse, Rat
WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~21kDa, endogenous levels of total Twist1 protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1336 was sufficient for detection of Twist in 10 µg of mouse muscle cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Nucleus
Subset of mesodermal cells.
TWIST1 is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. It has been implicated in cell lineage determination and differentiation. The strongest expression of this mRNA is in placental tissue, in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.
1. Reinhold M.I., Kapadia R.M., Liao Z., Naski M.S. (2006) J Biol Chem. 281(3): 1381-1388.
6/Sep/2016
NP_000465.1
7291
Q15672
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
