Immunogen
A synthesized peptide derived from human STAT3
Antibody's target
Human STAT3
Antibody's full description
Rabbit Anti-Human STAT3 Polyclonal
100 µl
SPC-1334D-ALP
350€
A synthesized peptide derived from human STAT3
Human STAT3
Rabbit Anti-Human STAT3 Polyclonal
Cell Signaling, Epigenetics, Cancer, Cardiovascular System, Heart
Polyclonal Antibodies
Acute Phase Response Factor Antibody, ADMIO Antibody, APRF Antibody, DNA binding protein APRF Antibody, HIES Antibody, Signal transducer and activator of transcription 3 Antibody, STAT 3 Antibody
WB, IHC
Rabbit
Human, Mouse, Rat
WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~85kDa, endogenous levels of total STAT3
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1334 was sufficient for detection of STAT3 in 10 µg of K562 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Nucleus
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Stat3 is an important transciption factor for many cytokines and growth factor receptors. It is constitutively activated in a number of human tumors and has oncogenic potential and anti-apoptotic activites.
1. Darnell J.E., et al. (1994) Science. 264: 1415-1421. 2. Garcia R. and Jove R. (1998) J Biomed Sci. 5: 79-85. 3. Bromberg J.F., et al. (1999) Cell. 98: 295-303.
6/Sep/2016
NP_644805.1
6774
P40763
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human K562 cell lysates showing detection of ~86kDa STAT3 protein using Rabbit Anti-STAT3 Polyclonal Antibody (SPC-1334). Lane 1: Human K562 whole cell lysates treated with the immunizing peptide. Lane 2: Human K562 whole cell lysates. Primary Antibody: Rabbit Anti-STAT3 Polyclonal Antibody (SPC-1334) at 1:1000. Predicted/Observed Size: ~86kDa. Rabbit Anti-STAT3 Antibody used in Western blot (WB) on K562 cell lysates (SPC-1334)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
