Immunogen
A synthesized peptide derived from human RhoA
Antibody's target
Human RhoA
Antibody's full description
Rabbit Anti-Human RhoA Polyclonal
100 µl
SPC-1332D-ALP
350€
A synthesized peptide derived from human RhoA
Human RhoA
Rabbit Anti-Human RhoA Polyclonal
Cell Signaling, Cancer
Polyclonal Antibodies
Aplysia ras related homolog 12 Antibody, ARH12 Antibody, ARHA Antibody, H12 Antibody, Ras homolog family member A Antibody, Rho A Antibody, RHO H12 Antibody, RHOA Antibody, Small GTP binding protein Rho A Antibody, Transforming protein Rho A Antibody
WB, IHC
Rabbit
Human, Mouse, Rat
WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~22kDa, endogenous levels of total RhoA
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1332 was sufficient for detection of RhoA in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cell Membrane, Cytoplasm, Cytoskeleton
See included datasheet or contact our support service.
RhoA is a small GTPase protein of the Rho family. The Rho family acts as molecular swithces and regulators of processes such as cell migration, proliferation and differentiation. RhoA specifically regulates actomyosin contractility, cytokinesis, focal adhesion assembly and cell polarity.
1. Bi D., et al. (2005) Circ. Res. 96: 890-897. 2. Kimura K., et al. (2000) J. Biol. Chem. 275: 17233-17236. 3. Barry S.T. and Critchley D.R. (1994) J. Cell Sci. 107 ( Pt 7): 2033-2045. 4. Van Keymeulen A., et al. (2006) J. Cell Biol. 174: 437-445.
6/Sep/2016
NP_001655.1
387
P61586
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HeLa cell lysates showing detection of ~22kDa RhoA protein using Rabbit Anti-RhoA Polyclonal Antibody (SPC-1332). Lane 1: Human HeLa treated with the immunizing peptide. Lane 2: Human HeLa. Primary Antibody: Rabbit Anti-RhoA Polyclonal Antibody (SPC-1332) at 1:1000. Predicted/Observed Size: ~22kDa. Rabbit Anti-RhoA Antibody used in Western blot (WB) on HeLa cell lysates (SPC-1332)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
