Immunogen
A synthesized peptide derived from human PTEN
Antibody's target
Human PTEN
Antibody's full description
Rabbit Anti-Human PTEN Polyclonal
A synthesized peptide derived from human PTEN
Human PTEN
Rabbit Anti-Human PTEN Polyclonal
Cell Signaling, Epigenetics, Cancer
Polyclonal Antibodies
GLM2 Antibody, MHAM Antibody, MMAC1 Antibody, Phosphatase and tensin like protein Antibody, Pten Antibody, PTEN1 Antibody, TEP1 Antibody
WB, IHC
Rabbit
Human, Mouse, Rat
WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~47kDa, endogenous levels of total PTEN
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1331 was sufficient for detection of PTEN in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Nucleus
Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
PTEN, also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers. It is a major negative regulator of the PI3K/Akt signaling pathway. It also regulates p53 protein levels and is involved in G protein-coupled signaling during chemotaxis.
1. Wan X. and Helman L.J. (2003) Oncogene. 22: 8205–8211. 2. Cantley L.C. and Neel B.G. (1999) Proc Natl Acad Sci USA. 96: 4240-4245. 3. Myers M.P., et al. (1998) Proc Natl Acad Sci USA. 95: 13513-13518. 4. Wu X., et al. (1998) Proc Natl Acad Sci USA. 95: 15587-15591. 5. Funamoto S., et al. (2002) Cell. 109: 611-623. 6. Iijima M. and Devreotes P. (2002) Cell. 109: 599-610.
6/Sep/2016
NP_000305.3
5728
P60484
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HeLa cell lysates showing detection of ~54kDa PTEN protein using Rabbit Anti-PTEN Polyclonal Antibody (SPC-1331). Lane 1: Vanadate treated Human HeLa whole cell lysates treated with the immunizing peptide. Lane 2: Vanadate treated Human HeLa whole cell lysates. Primary Antibody: Rabbit Anti-PTEN Polyclonal Antibody (SPC-1331) at 1:1000. Predicted/Observed Size: ~54kDa. Rabbit Anti-PTEN Antibody used in Western blot (WB) on HeLa cell lysates (SPC-1331)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Anti-FITC Antibody
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
