Immunogen
Synthesized peptide derived from human MDM2.
Antibody's target
Human MDM2
Antibody's full description
Rabbit Anti-Human MDM2 Polyclonal
100 µl
SPC-1329D-ALP
350€
Synthesized peptide derived from human MDM2.
Human MDM2
Rabbit Anti-Human MDM2 Polyclonal
Polyclonal Antibodies
ACTFS antibody, Double minute 2 protein antibody, E3 ubiquitin-protein ligase Mdm2 antibody, Hdm 2 antibody, Hdm2 antibody, HDMX antibody, MDM 2 antibody, MDM2 antibody, MDM2 oncogene E3 ubiquitin protein ligase antibody, Mdm2 p53 E3 ubiquitin protein ligase homolog antibody, Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody, MDM2_HUMAN antibody, MDM2BP antibody, Mouse Double Minute 2 antibody, MTBP antibody, Murine Double Minute Chromosome 2 antibody, Oncoprotein Mdm2 antibody, p53 Binding Protein Mdm2 antibody, p53-binding protein Mdm2 antibody, Ubiquitin protein ligase E3 Mdm2 antibody, Ubiquitin protein ligase E3 Mdm2 antibody
WB, IHC, ICC/IF, ELISA
Rabbit
Human, Rat
WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:40000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of total MDM2 protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1329 was sufficient for detection of MDM2 in 10 µg of SKOV3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Nucleus, Cytoplasm
See included datasheet or contact our support service.
Mouse double minute 2 homolog (MDM2) also known as E3 ubiquitin-protein ligase Mdm2 is a protein that in humans is encoded by the MDM2 gene. MDM2 is a target of the transcription factor tumor protein p53. This protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
1. Uhrinova S et al. (2005) J. Mol. Biol. 350 (3): 587–98. 2. Oliner JD et al. (1992) Nature 358 (6381): 80–3. 3. Wade M. et al. (2006) J. Biol. Chem. 281 (44): 33036–44. 4. Ebrahim, M. et al. (2015) Histology and histopathology, 30(11), 1271-1282. 30(11):1271-1282.
6/Sep/2016
NP_002383.2
4193
Q00987
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Immunohistochemistry analysis using Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329). Tissue: A549. Species: Human. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human SKOV3 cell lysates showing detection of ~90kDa MDM2 protein using Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329). Lane 1: Human SKOV3 cells. Lane 2: Human SKOV3 cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329) at 1:1000. Predicted/Observed Size: ~90kDa. Rabbit Anti-MDM2 Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1329) | Rabbit Anti-MDM2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on A549 (SPC-1329) | Rabbit Anti-MDM2 Antibody used in Western blot (WB) on SKOV3 cell lysates (SPC-1329)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
