Immunogen
Synthesized peptide derived from human MCL1.
Antibody's target
Human MCL1
Antibody's full description
Rabbit Anti-Human MCL1 Polyclonal
100 µl
SPC-1328D-ALP
350€
Synthesized peptide derived from human MCL1.
Human MCL1
Rabbit Anti-Human MCL1 Polyclonal
Polyclonal Antibodies
Bcl 2 related protein EAT/mcl1 antibody, Bcl-2-like protein 3 antibody, Bcl-2-related protein EAT/mcl1 antibody, BCL2 related antibody, Bcl2-L-3 antibody, BCL2L3 antibody, EAT antibody, Induced myeloid leukemia cell differentiation protein Mcl 1 antibody, Induced myeloid leukemia cell differentiation protein Mcl-1 antibody, MCL 1 antibody, MCL1 antibody, MCL1-ES antibody, mcl1/EAT antibody, MCL1_HUMAN antibody, MCL1L antibody, MCL1S antibody, MGC104264 antibody, MGC1839 antibody, Myeloid Cell Leukemia 1 antibody, Myeloid cell leukemia ES antibody, Myeloid cell leukemia sequence 1 antibody, Myeloid cell leukemia sequence 1 BCL2 related antibody, Myeloid cell leukemia sequence 1 isoform 1 antibody, OTTHUMP00000032794 antibody, OTTHUMP00000032795 antibody, TM antibody
WB, IHC, ELISA
Rabbit
Human, Mouse, Rat
WB (1:1000), IHC (1:100), ELISA (1:40000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of total MCL1 protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1328 was sufficient for detection of MCL1 in 10 µg of HuvEc cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Membrane, Cytoplasm, Mitochondrion, Nucleus
See included datasheet or contact our support service.
Induced myeloid leukemia cell differentiation protein Mcl-1 is a protein that in humans is encoded by the MCL1 gene. It is involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
1. Kozopas KM. et al. (1993) Proc Natl Acad Sci U S A 90 (8): 3516–20. 2. Craig RW. et al. (1995) Genomics 23 (2): 457–63.
6/Sep/2016
NP_068779.1
4170
Q07820
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Immunohistochemistry analysis using Rabbit Anti-MCL1 Polyclonal Antibody (SPC-1328). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-MCL1 Polyclonal Antibody (SPC-1328) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human HuvEc cell lysates showing detection of ~40kDa MCL1 protein using Rabbit Anti-MCL1 Polyclonal Antibody (SPC-1328). Lane 1: Human HuvEc cells. Lane 2: Human HuvEc cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-MCL1 Polyclonal Antibody (SPC-1328) at 1:1000. Predicted/Observed Size: ~40kDa. Rabbit Anti-MCL1 Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1328) | Rabbit Anti-MCL1 Antibody used in Western blot (WB) on HuvEc cell lysates (SPC-1328)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
