Immunogen
Synthesized peptide derived from C-terminal of human ki67.
Antibody's target
Human Ki67
Antibody's full description
Rabbit Anti-Human Ki67 Polyclonal
100 µl
SPC-1327D-ALP
350€
Synthesized peptide derived from C-terminal of human ki67.
Human Ki67
Rabbit Anti-Human Ki67 Polyclonal
Polyclonal Antibodies
Antigen identified by monoclonal antibody Ki 67 antibody, Antigen KI-67 antibody, Antigen KI67 antibody, KI67_HUMAN antibody, KIA antibody, Marker of proliferation Ki-67 antibody, MIB 1 antibody, MIB antibody, MKI67 antibody, PPP1R105 antibody, Proliferation related Ki 67 antigen antibody, Protein phosphatase 1 regulatory subunit 105 antibody, RP11-380J17.2 antibody
IHC, ICC/IF, ELISA
Rabbit
Human
IHC (1:100), ICC/IF (1:500), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of total ki67protein.
-20°C
Blue Ice or 4°C
A 1:100 dilution of SPC-1327 was sufficient for detection of Ki67 in human lung carcinoa tissue using Goat Anti-Rabbit IgG as the secondary antibody.
Nucleus, Chromosome
See included datasheet or contact our support service.
Antigen KI-67 also known as Ki-67 or MKI67 is a protein that in humans is encoded by the MKI67 gene. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore, it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. Due to its association with cell divison process, Ki-67 is routinely used as cellular proliferation marker of solid tumors as well as certain hematological malignancies, and a correlation has been demonstrated between Ki-67 index and the histopathological grade of cancers.
1. Schonk DM. et al. (1989) Hum. Genet. 83 (3): 297–9. 2. Bullwinkel J. et al. (2006) J. Cell. Physiol. 206 (3): 624–35. 3. Rahmanzadeh R. et al. (2007) Cell Prolif. 40 (3): 422–30. 4. Scholzen T and Gerdes J (2000) J. Cell. Physiol. 182 (3): 311–22.
6/Sep/2016
NP_002408.3
4288
P46013
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Immunohistochemistry analysis using Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327). Tissue: Cos7. Species: Monkey. Primary Antibody: Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327) at 1:100. The image on the right is treated with the synthesized peptide. Rabbit Anti-Ki67 Antibody used in Immunohistochemistry (IHC) on Lung Carcinoma Tissue (SPC-1327) | Rabbit Anti-Ki67 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Cos7 (SPC-1327)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
