Immunogen
Synthesized peptide derived from human iNOS.
Antibody's target
Human iNOS
Antibody's full description
Rabbit Anti-Human iNOS Polyclonal
100 µl
SPC-1325D-ALP
350€
Synthesized peptide derived from human iNOS.
Human iNOS
Rabbit Anti-Human iNOS Polyclonal
Polyclonal Antibodies
HEP-NOS antibody, Hepatocyte NOS antibody, HEPNOS antibody, inducible antibody, Inducible nitric oxide synthase antibody, Inducible NO synthase antibody, Inducible NOS antibody, iNOS antibody, MAC NOS antibody, Macrophage NOS antibody, Nitric oxide synthase 2 inducible antibody, Nitric oxide synthase 2 inducible macrophage antibody, nitric oxide synthase 2A (inducible, hepatocytes) antibody, Nitric oxide synthase antibody, Nitric oxide synthase inducible antibody, nitric oxide synthase, macrophage antibody, NOS 2 antibody, NOS antibody, Nos II antibody, NOS type II antibody, nos2 antibody, NOS2_HUMAN antibody, NOS2A antibody, NOS2A, Inducible, Hepatocyte antibody, Peptidyl-cysteine S-nitrosylase NOS2 antibody
WB, IHC, ICC/IF, ELISA
Rabbit
Human
WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of total iNOS protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1325 was sufficient for detection of iNOS in 10 µg of NIH/3T3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Nucleus, Peroxisome
See included datasheet or contact our support service.
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned.
1. Knowles RG and Moncada S. (1994) Biochem. J. 298 (2): 249–58. 2. Burger DE. et al. (2009) Circulation 120 (14): 1345–54. 3. Taylor BS. (1997) Arch Surg 132 (11): 1177–83.
6/Sep/2016
NP_000616.3
4843
P35228
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Immunohistochemistry analysis using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Tissue: HepG2 cells. Species: Human. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Mouse NIH/3T3 cell lysates showing detection of ~131kDa iNOS protein using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Lane 1: Mouse NIH/3T3. Lane 2: Mouse NIH/3T3 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:1000. Predicted/Observed Size: ~131kDa. Rabbit Anti-iNOS Antibody used in Immunohistochemistry (IHC) on Lung Carcinoma Tissue (SPC-1325) | Rabbit Anti-iNOS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on HepG2 cells (SPC-1325) | Rabbit Anti-iNOS Antibody used in Western blot (WB) on NIH/3T3 cell lysates (SPC-1325)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
