Immunogen
Synthesized peptide derived from human HDAC1.
Antibody's target
Human HDAC1
Antibody's full description
Rabbit Anti-Human HDAC1 Polyclonal
100 µl
SPC-1324D-ALP
350€
Synthesized peptide derived from human HDAC1.
Human HDAC1
Rabbit Anti-Human HDAC1 Polyclonal
Polyclonal Antibodies
DKFZp686H12203 antibody, GON 10 antibody, HD1 antibody, HDAC 1 antibody, HDAC1 antibody, HDAC1_HUMAN antibody, Histone deacetylase 1 antibody, Reduced potassium dependency yeast homolog like 1 antibody, RPD3 antibody, RPD3L1 antibody
WB, IHC, ICC/IF, ELISA
Rabbit
Human, Mouse, Rat
WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of total HDAC1 protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1324 was sufficient for detection of HDAC1 in 10 µg of NIH/3T3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Nucleus
See included datasheet or contact our support service.
Histone deacetylase 1 is an enzyme that in humans is encoded by the HDAC1 gene. Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
1. Taunton J. et al. (1996) Science 272 (5260): 408–11. 2. Gerdin AK. (2010) Acta Ophthalmologica 88 (S248).
6/Sep/2016
NP_004955.2
3065
Q13547
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Immunohistochemistry analysis using Rabbit Anti-HDAC1 Polyclonal Antibody (SPC-1324). Tissue: Lung Carcinoma Tissue. Species: Human. Primary Antibody: Rabbit Anti-HDAC1 Polyclonal Antibody (SPC-1324) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HDAC1 Polyclonal Antibody (SPC-1324). Tissue: NIH/3T3. Species: Mouse. Primary Antibody: Rabbit Anti-HDAC1 Polyclonal Antibody (SPC-1324) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Mouse NIH/3T3 cell lysates showing detection of ~62kDa HDAC1 protein using Rabbit Anti-HDAC1 Polyclonal Antibody (SPC-1324). Lane 1: Mouse NIH/3T3. Lane 2: Mouse NIH/3T3 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-HDAC1 Polyclonal Antibody (SPC-1324) at 1:1000. Predicted/Observed Size: ~62kDa. Rabbit Anti-HDAC1 Antibody used in Immunohistochemistry (IHC) on Lung Carcinoma Tissue (SPC-1324) | Rabbit Anti-HDAC1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on NIH/3T3 (SPC-1324) | Rabbit Anti-HDAC1 Antibody used in Western blot (WB) on NIH/3T3 cell lysates (SPC-1324)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
