Immunogen
Peptide sequence around aa.273~277(T-I-L-V-E)derived from Human DDX4.
Antibody's target
Human DDX4
Antibody's full description
Rabbit Anti-Human DDX4 Polyclonal
100 µl
SPC-1322D-ALP
350€
Peptide sequence around aa.273~277(T-I-L-V-E)derived from Human DDX4.
Human DDX4
Rabbit Anti-Human DDX4 Polyclonal
Polyclonal Antibodies
DDX 4 antibody, Ddx4 antibody, DDX4_HUMAN antibody, DEAD (Asp Glu Ala Asp) box polypeptide 4 antibody, DEAD box protein 4 antibody, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody, MVH antibody, Probable ATP dependent RNA helicase DDX4 antibody, Probable ATP-dependent RNA helicase DDX4 antibody, VASA antibody, Vasa homolog antibody
WB
Rabbit
Human, Mouse, Rat
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous level of total DDX4 protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1322 was sufficient for detection of DDX4 in 10 µg of HeLa, K562, 3T3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Perinuclear Region
See included datasheet or contact our support service.
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development.
1. Castrillon D.H. et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97:9585-9590. 2. Schmutz J. et al. (2004) Nature 431:268-274.
6/Sep/2016
NP_077726.1
54514
Q9NQI0
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human, Mouse HeLa, K562, 3T3 cell lysates showing detection of ~80kDa DDX4 protein using Rabbit Anti-DDX4 Polyclonal Antibody (SPC-1322). Lane 1: Human HeLa. Lane 2: Human K562. Lane 3: Mouse 3T3. Primary Antibody: Rabbit Anti-DDX4 Polyclonal Antibody (SPC-1322) at 1:1000. Predicted/Observed Size: ~80kDa. Rabbit Anti-DDX4 Antibody used in Western blot (WB) on HeLa, K562, 3T3 cell lysates (SPC-1322)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus