Immunogen
A synthesized peptide derived from human COX IV
Antibody's target
Human COX IV
Antibody's full description
Rabbit Anti-Human COX IV Polyclonal
100 µl
SPC-1320D-ALP
350€
A synthesized peptide derived from human COX IV
Human COX IV
Rabbit Anti-Human COX IV Polyclonal
Polyclonal Antibodies
COX IV-1 antibody, COX4 antibody, COX4-1 antibody, COX41_HUMAN antibody, COX4I1 antibody, COXIV antibody, Cytochrome c oxidase polypeptide IV antibody, Cytochrome c oxidase subunit 4 isoform 1 antibody, Cytochrome C Oxidase Subunit IV antibody, Cytochrome c oxidase subunit IV isoform 1 antibody, mitochondrial antibody
WB, IHC, ICC/IF
Rabbit
Human, Mouse, Rat
WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of total COX IV
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1320 was sufficient for detection of COX IV in 10 µg of Jurkat cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Mitochondrion, Mitochondrion inner membrane
See included datasheet or contact our support service.
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it.
1. Zeviani M. (1987) Gene 55:205-217. 2. Lomax M.I. et al. (1990) Gene 86:209-216. 3. Ota T. et al. (2004) Nat. Genet. 36:40-45.
6/Sep/2016
NP_001852.1
1327
P13073
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human Jurkat cell lysates showing detection of ~17kDa COX IV protein using Rabbit Anti-COX IV Polyclonal Antibody (SPC-1320). Lane 1: Human Jurkat cell lysate treated with the immunizing peptide. Lane 2: Human Jurkat cell lysate. Primary Antibody: Rabbit Anti-COX IV Polyclonal Antibody (SPC-1320) at 1:1000. Predicted/Observed Size: ~17kDa. Rabbit Anti-COX IV antibody used in Western blot (WB) on Jurkat cell lysates (SPC-1320)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus