Immunogen
Synthesized peptide derived from human Caspase 3.
Antibody's target
Human Caspase 3 (cleaved)
Antibody's full description
Rabbit Anti-Human Caspase 3 (cleaved) Polyclonal
100 µl
SPC-1319D-ALP
350€
Synthesized peptide derived from human Caspase 3.
Human Caspase 3 (cleaved)
Rabbit Anti-Human Caspase 3 (cleaved) Polyclonal
Polyclonal Antibodies
Apopain antibody, CASP-3 antibody, CASP3 antibody, CASP3_HUMAN antibody, Caspase 3 antibody, caspase 3, apoptosis-related cysteine protease antibody, Caspase-3 subunit p12 antibody, CPP 32 antibody, CPP-32 antibody, Cysteine protease CPP32 antibody, PARP cleavage protease antibody, Procaspase 3 antibody, Protein Yama antibody, SCA-1 antibody, SCA1 antibody, SREBP cleavage activity 1 antibody, Yama antibody, Yama protein antibody
WB, IHC
Rabbit
Human, Mouse, Rat
WB (1:2000), IHC (200); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1319 was sufficient for detection of Caspase 3 in 10 µg of COLO cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cytoplasm
See included datasheet or contact our support service.
Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Caspase-3 is activated in the apoptotic cell both by extrinsic (death ligand) and intrinsic (mitochondrial) pathways. The zymogen feature of caspase-3 is necessary because if unregulated, caspase activity would kill cells indiscriminately. Caspase-3 has been found to be necessary for normal brain development as well as its typical role in apoptosis, where it is responsible for chromatin condensation and DNA fragmentation. Elevated levels of a fragment of Caspase-3, p17, in the bloodstream is a sign of a recent myocardial infarction. It is now being shown that caspase-3 may play a role in embryonic and hematopoietic stem cell differentiation.
1. Alnemri ES. et al. (1996) Cell 87 (2): 171. 2. Salvesen GS. (2002) Cell Death Differ. 9 (1): 3–5. 3. Ghavami S (2009) J. Med. Genet. 46 (8): 497–510. 4. Porter AG and Jänicke RU (1999) Cell Death Differ. 6 (2): 99–104. 5. Agosto M (2011) J. Am. Coll. Cardiol. 57 (2): 220–1. 6. Abdul-Ghani M. (2008) Cell Stem Cell 2 (6): 515–6.
6/Sep/2016
NP_116786.1
836
P42574
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Immunohistochemistry analysis using Rabbit Anti-Caspase 3 Polyclonal Antibody (SPC-1319). Tissue: Ehrlich Ascites. Species: Mouse. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Caspase 3 Polyclonal Antibody (SPC-1319) at 1:100. | Western blot analysis of Human COLO cell lysates showing detection of ~17kDa Caspase 3 protein using Rabbit Anti-Caspase 3 Polyclonal Antibody (SPC-1319). Lane 1: Human COLO cell lysate treated with the immunizing peptide. Lane 2: Human COLO cell lysate. Primary Antibody: Rabbit Anti-Caspase 3 Polyclonal Antibody (SPC-1319) at 1:1000. Predicted/Observed Size: ~17kDa. Rabbit Anti-Caspase 3 Antibody (cleaved) used in Immunohistochemistry (IHC) on Ehrlich Ascites (SPC-1319) | Rabbit Anti-Caspase 3 Antibody (cleaved) used in Western blot (WB) on COLO cell lysates (SPC-1319)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
