Immunogen
Synthesized unphosphorylated peptide derived from human β-catenin around the phosphorylation site of tyrosine 654 (A-T-YP-A-A).
Antibody's target
Human beta Catenin
Antibody's full description
Rabbit Anti-Human beta Catenin Polyclonal
100 µl
SPC-1318D-ALP
350€
Synthesized unphosphorylated peptide derived from human β-catenin around the phosphorylation site of tyrosine 654 (A-T-YP-A-A).
Human beta Catenin
Rabbit Anti-Human beta Catenin Polyclonal
Polyclonal Antibodies
Beta catenin antibody, Beta-catenin antibody, Cadherin associated protein antibody, Catenin (cadherin associated protein), beta 1, 88kDa antibody, Catenin beta 1 antibody, Catenin beta-1 antibody, CATNB antibody, CHBCAT antibody, CTNB1_HUMAN antibody, CTNNB antibody, CTNNB1 antibody, DKFZp686D02253 antibody, FLJ25606 antibody, FLJ37923 antibody, OTTHUMP00000162082 antibody, OTTHUMP00000165222 antibody, OTTHUMP00000165223 antibody, OTTHUMP00000209288 antibody, OTTHUMP00000209289 antibody
WB
Rabbit
Human, Mouse, Rat
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of totalβ-catenin protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1318 was sufficient for detection of beta Catenin in 10 µg of SW626 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Cytoskeleton, Nucleus, Cell Junction, Cell Membrane
See included datasheet or contact our support service.
Catenin beta-1 also known as β-catenin is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is the first characterized member of the β -catenin family. β-catenin contains 12 ARM repeats and has a dual function, depending on its localization. Cytoplasmic β-catenin is a critical component of E-cadherin/catenin adhesion complexes and regulates cadherin-dependent cell-cell interactions. When localized to the nucleus, β -catenin regulates binding to transcription factors from the TCF/LEF family, activating transcription. β -catenin is expressed in hair follicle cells such as those found in the inner and outer portions of the root sheaths, and the basal and peripheral matrix cells. Expression has also been observed in colon tissue. Defects in expression have been linked to the development of several tumors and cancers.
1. Kraus C. et al. (1994) Genomics 23 (1): 272–4. 2. MacDonald BT et al. (2009) Developmental Cell 17 (1): 9–26. 3. Peifer M. et al. (1991) Development 111 (4): 1029–1043. 4. Noordermeer J. (1994) Nature 367 (6458): 80–83.
6/Sep/2016
NP_001895.1
1499
P35222
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human SW626 cell lysates showing detection of ~94kDa beta Catenin protein using Rabbit Anti-beta Catenin Polyclonal Antibody (SPC-1318). Primary Antibody: Rabbit Anti-beta Catenin Polyclonal Antibody (SPC-1318) at 1:1000. Predicted/Observed Size: ~94kDa. Rabbit Anti-beta Catenin Antibody used in Western blot (WB) on SW626 cell lysates (SPC-1318)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus