Immunogen
Synthesized peptide derived from internal of human TNFA.
Antibody's target
Human TNF alpha
Antibody's full description
Rabbit Anti-Human TNF alpha Polyclonal
100 µl
SPC-1315D-ALP
350€
Synthesized peptide derived from internal of human TNFA.
Human TNF alpha
Rabbit Anti-Human TNF alpha Polyclonal
Polyclonal Antibodies
APC1 Antibody, APC1 protein Antibody, Cachectin Antibody, DIF Antibody, Differentiation inducing factor Antibody, Macrophage cytotoxic factor Antibody, TNF alpha Antibody, Tnf Antibody, TNF superfamily, member 2 Antibody, TNF, macrophage derived Antibody, TNF, monocyte derived Antibody, TNF-a Antibody, TNF-alpha Antibody, TNFA Antibody, TNFA_HUMAN Antibody, TNFSF2 Antibody, Tumor necrosis factor alpha Antibody, Tumor necrosis factor Antibody, Tumor necrosis factor ligand superfamily member 2 Antibody, Tumor Necrosis Factor, Membrane Form Antibody, Tumor necrosis factor, soluble form Antibody
IHC, ICC/IF, ELISA
Rabbit
Human, Mouse, Rat
IHC (1:100), ICC/IF (1:500), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of total TNFA protein.
-20°C
Blue Ice or 4°C
A 1:100 dilution of SPC-1315 was sufficient for detection of TNF alpha in human HepG2 cells using Goat Anti-Rabbit IgG as the secondary antibody.
Cell Membrane
See included datasheet or contact our support service.
Tumor necrosis factor is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. The primary role of TNF is in the regulation of immune cells. TNF, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, cachexia, inflammation and to inhibit tumorigenesis and viral replication and respond to sepsis via IL1 & IL6 producing cells. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression, Psoriasis and inflammatory bowel disease (IBD).
1. Swardfager W et al. (2010) Biol Psychiatry 68 (10): 930–941. 2. Locksley RM et al. (2001) Cell 104 (4): 487–501. 3. Dowlati Y et al. (2010) Biol Psychiatry 67 (5): 446–457. 4. Victor FC and Gottlieb AB (2002) J Drugs Dermatol 1 (3): 264–75. 5. Brynskov J. (2002) Gut 51 (1): 37–43.
6/Sep/2016
NP_000585.2
7124
P01375
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Immunohistochemistry analysis using Rabbit Anti-TNF alpha Polyclonal Antibody (SPC-1315). Tissue: Colon Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-TNF alpha Polyclonal Antibody (SPC-1315) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF alpha Polyclonal Antibody (SPC-1315). Tissue: HepG2 cells. Species: Human. Primary Antibody: Rabbit Anti-TNF alpha Polyclonal Antibody (SPC-1315) at 1:100. The image on the right is treated with the synthesized peptide. Rabbit Anti-TNF alpha Antibody used in Immunohistochemistry (IHC) on Colon Carcinoma Tissue (SPC-1315) | Rabbit Anti-TNF alpha Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on HepG2 cells (SPC-1315)
Non-hazardous
Canada
1.4
0.1
In Stock
The Rabbit Anti- TNF alpha Polyclonal: Alkaline Phosphatase is a α- or alpha protein sometimes glycoprotein present in blood.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.
