Immunogen
Synthesized peptide derived from human Thyroid Hormone Receptor β antibody.
Antibody's target
Human THRB
Antibody's full description
Rabbit Anti-Human THRB Polyclonal
100 µl
SPC-1314D-ALP
350€
Synthesized peptide derived from human Thyroid Hormone Receptor β antibody.
Human THRB
Rabbit Anti-Human THRB Polyclonal
Polyclonal Antibodies
Avian erythroblastic leukemia viral (v erb a) oncogene homolog 2 Antibody, C ERBA 2 Antibody, C ERBA BETA Antibody, c-erbA-2 Antibody, c-erbA-beta Antibody, ERBA 2 Antibody, ERBA BETA Antibody, ERBA2 Antibody, Erythroblastic leukemia viral (v erb a) oncogene homolog 2 avian Antibody, generalized resistance to thyroid hormone Antibody, GRTH Antibody, MGC126109 Antibody, MGC126110 Antibody, NR1A2 Antibody, Nuclear receptor subfamily 1 group A member 2 Antibody, Oncogene ERBA2 Antibody, PRTH Antibody, THB_HUMAN Antibody, THR1 Antibody, THRB 1 Antibody, THRB 2 Antibody, thrB Antibody, THRB1 Antibody, THRB2 Antibody, Thyroid hormone nuclear receptor beta variant 1 Antibody, Thyroid hormone receptor beta 1 Antibody, Thyroid hormone receptor beta 2 Antibody, Thyroid hormone receptor beta Antibody, Thyroid hormone receptor, beta (erythroblastic leukemia viral (v erb a) oncogene homolog 2, avian) Antibody
WB, IHC, ELISA
Rabbit
Human, Mouse, Rat
WB (1:1000), IHC (1:100), ELISA (1:5000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~42kDa, endogenous levels of total Thyroid Hormone Receptor β protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1314 was sufficient for detection of THRB in 10 µg of LOVO cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Nucleus
See included datasheet or contact our support service.
There are three forms of the thyroid hormone receptor designated alpha-1, beta-1 and beta-2 that are able to bind thyroid hormone. There are two TR-α receptor splice variants encoded by the THRA gene and two TR-β isoform splice variants encoded by the THRB gene. Thyroid hormone receptor β-1 operates as a hormone-dependent transcriptional regulator that binds DNA sequences and is activated by 3,5,3’-l-triiodothyronine (T3). Thyroid hormone receptor β-1 is important in vertebrate development, specifically in developing limbs and face. 9-cis-retinoic acid receptor (RXR) and retinoic acid receptor (RAR) receptor families are often associated with thyroid hormone receptor β-1 and form homodimers and heterodimers.
1. Flamant F et al. (2006) Pharmacol Rev 58 (4): 705–11.
6/Sep/2016
NP_000452.2
7068
P10828
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Immunohistochemistry analysis using Rabbit Anti-THRB Polyclonal Antibody (SPC-1314). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-THRB Polyclonal Antibody (SPC-1314) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human LOVO cell lysates showing detection of ~42kDa THRB protein using Rabbit Anti-THRB Polyclonal Antibody (SPC-1314). Lane 1: Human LOVO cells. Lane 2: Human LOVO cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-THRB Polyclonal Antibody (SPC-1314) at 1:1000. Predicted/Observed Size: ~42kDa. Other Band(s): ~55kDa. Rabbit Anti-THRB Antibody used in Immunohistochemistry (IHC) on Lung Carcinoma Tissue (SPC-1314) | Rabbit Anti-THRB Antibody used in Western blot (WB) on LOVO cell lysates (SPC-1314)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
