Immunogen
Synthesized peptide derived from human TGF β3.
Antibody's target
Human TGF beta 3
Antibody's full description
Rabbit Anti-Human TGF beta 3 Polyclonal
100 µl
SPC-1313D-ALP
350€
Synthesized peptide derived from human TGF β3.
Human TGF beta 3
Rabbit Anti-Human TGF beta 3 Polyclonal
Polyclonal Antibodies
ARVD Antibody, ARVD1 Antibody, FLJ16571 Antibody, MGC105479 Antibody, MGC118722 Antibody, prepro-transforming growth factor beta-3 Antibody, RNHF Antibody, TGF beta 3 Antibody, TGF beta3 Antibody, TGF-beta-3 Antibody, TGFB 3 Antibody, Tgfb3 Antibody, TGFB3_HUMAN Antibody, transforming growth factor beta 3 Antibody, Transforming growth factor beta-3 Antibody
WB, IHC, ELISA
Rabbit
Human, Mouse, Rat
WB (1:1000), IHC (1:100), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~50kDa, endogenous levels of total TGF β3 protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1313 was sufficient for detection of TGF beta 3 in 10 µg of Cos7 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Secreted
See included datasheet or contact our support service.
Transforming growth factor beta-3 is a protein that in humans is encoded by the TGFB3 gene. It is a membrane proteoglycan that often functions as a co-receptor with other TGF-beta receptor superfamily members. Ectodomain shedding produces soluble TGFBR3, which may inhibit TGFB signaling. Decreased expression of this receptor has been observed in various cancers. TGFBR3 is extensively modified by glycosaminoglycan groups (GAG). TGF-β3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-β3, mammals develop a deformity known as a cleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse.
1. Bandyopadhyay B et al. (2006) The Journal of Cell Biology 172 (7): 1093–105. 2. Herpin A et al. (2004) Developmental and Comparative Immunology 28 (5): 461–85. 3. Taya Y et al. (1999) Development 126 (17): 3869–79. 4. Dudas M et al. (2004) Developmental Biology 266 (1): 96–108. 5. Kaartinen V et al. (1995) Nature Genetics 11 (4): 415–21.
6/Sep/2016
NP_003230.1
7043
P10600
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Immunohistochemistry analysis using Rabbit Anti-TGF beta 3 Polyclonal Antibody (SPC-1313). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-TGF beta 3 Polyclonal Antibody (SPC-1313) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Monkey Cos7 cell lysates showing detection of ~12.5kDa TGF beta 3 protein using Rabbit Anti-TGF beta 3 Polyclonal Antibody (SPC-1313). Lane 1: Monkey Cos7 cells. Lane 2: Monkey Cos7 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-TGF beta 3 Polyclonal Antibody (SPC-1313) at 1:1000. Predicted/Observed Size: ~12.5kDa . Rabbit Anti-TGF beta 3 Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1313) | Rabbit Anti-TGF beta 3 Antibody used in Western blot (WB) on Cos7 cell lysates (SPC-1313)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
