Immunogen
Synthesized unphosphorylated peptide derived from human PPAR α around the phosphorylation site of serine 21 (L-E-SP-P-L).
Antibody's target
Human PPAR alpha
Antibody's full description
Rabbit Anti-Human PPAR alpha Polyclonal
100 µl
SPC-1309D-ALP
350€
Synthesized unphosphorylated peptide derived from human PPAR α around the phosphorylation site of serine 21 (L-E-SP-P-L).
Human PPAR alpha
Rabbit Anti-Human PPAR alpha Polyclonal
Polyclonal Antibodies
hPPAR Antibody, MGC2237 Antibody, MGC2452 Antibody, NR1C1 Antibody, Nuclear receptor subfamily 1 group C member 1 Antibody, OTTHUMP00000197740 Antibody, OTTHUMP00000197741 Antibody, Peroxisome proliferative activated receptor alpha Antibody, Peroxisome proliferator activated receptor alpha Antibody, Peroxisome proliferator-activated receptor alpha Antibody, PPAR Antibody, PPAR-alpha Antibody, ppara Antibody, PPARA_HUMAN Antibody, PPARalpha Antibody
WB, ICC/IF, ELISA
Rabbit
Human
WB (1:1000), ICC/IF (1:500), ELISA (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~52kDa, endogenous levels of total PPAR α protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1309 was sufficient for detection of PPAR alpha in 10 µg of NIH/3T3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Nucleus
Skeletal muscle, liver, heart and kidney.
Peroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. PPARG is mainly present in adipose tissue, colon and macrophages. Two isoforms of PPARG are detected in the human and in the mouse: PPAR-γ1 (found in nearly all tissues except muscle) and PPAR-γ2 (mostly found in adipose tissue and the intestine)
1. Chandra, V. et al. (2008) Nature 456 (7220): 350–356. 2. Greene ME et al. (1995) Gene Expr. 4 (4–5): 281–99. 3. Elbrecht A. et al. (1996) Biochem. Biophys. Res. Commun. 224 (2): 431–7.
6/Sep/2016
NP_005027.2
5465
Q07869
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309). Tissue: HeLa. Species: Human. Primary Antibody: Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Mouse NIH/3T3 cell lysates showing detection of ~52kDa PPAR alpha protein using Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309). Lane 1: Mouse NIH/3T3. Lane 2: Mouse NIH/3T3 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309) at 1:1000. Predicted/Observed Size: ~52kDa. Rabbit Anti-PPAR alpha Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on HeLa (SPC-1309) | Rabbit Anti-PPAR alpha Antibody used in Western blot (WB) on NIH/3T3 cell lysates (SPC-1309)
Non-hazardous
Canada
1.4
0.1
In Stock
The Rabbit Anti- PPAR alpha Polyclonal: Alkaline Phosphatase is a α- or alpha protein sometimes glycoprotein present in blood.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
