Immunogen
A synthesized peptide derived from human PPAR-gamma
Antibody's target
Human PPAR gamma
Antibody's full description
Rabbit Anti-Human PPAR gamma Polyclonal
100 µl
SPC-1308D-ALP
350€
A synthesized peptide derived from human PPAR-gamma
Human PPAR gamma
Rabbit Anti-Human PPAR gamma Polyclonal
Polyclonal Antibodies
CIMT1 Antibody, GLM1 Antibody, NR1C3 Antibody, Nuclear receptor subfamily 1 group C member 3 Antibody, OTTHUMP00000185032 Antibody, OTTHUMP00000185036 Antibody, Peroxisome proliferator activated nuclear receptor gamma variant 1 Antibody, Peroxisome proliferator activated receptor gamma 1 Antibody, Peroxisome Proliferator Activated Receptor gamma Antibody, Peroxisome proliferator-activated receptor gamma Antibody, PPAR gamma Antibody, PPAR-gamma Antibody, PPARG Antibody, PPARG_HUMAN Antibody, PPARG1 Antibody, PPARG2 Antibody, PPARgamma Antibody
WB, IHC
Rabbit
Human, Mouse, Rat
WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~56kDa, endogenous levels of total PPAR-gamma
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1308 was sufficient for detection of PPAR gamma in 10 µg of HuvEc cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Nucleus, Cytoplasm
Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary
Peroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. PPARG is mainly present in adipose tissue, colon and macrophages. Two isoforms of PPARG are detected in the human and in the mouse: PPAR-γ1 (found in nearly all tissues except muscle) and PPAR-γ2 (mostly found in adipose tissue and the intestine)
1. Chandra, V. et al. (2008) Nature 456 (7220): 350–356. 2. Greene ME et al. (1995) Gene Expr. 4 (4–5): 281–99. 3. Elbrecht A. et al. (1996) Biochem. Biophys. Res. Commun. 224 (2): 431–7.
6/Sep/2016
NP_056953.2
5468
P37231
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HuvEc cell lysates showing detection of ~57kDa PPAR gamma protein using Rabbit Anti-PPAR gamma Polyclonal Antibody (SPC-1308). Lane 1: Human HuvEc Whole Cell lysates treated with the immunizing peptide. Lane 2: Human HuvEc Whole Cell lysates. Primary Antibody: Rabbit Anti-PPAR gamma Polyclonal Antibody (SPC-1308) at 1:1000. Predicted/Observed Size: ~57kDa. Rabbit Anti-PPAR gamma Antibody used in Western blot (WB) on HuvEc cell lysates (SPC-1308)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus