Immunogen
Synthesized peptide derived from human PCNA.
Antibody's target
Human PCNA
Antibody's full description
Rabbit Anti-Human PCNA Polyclonal
100 µl
SPC-1307D-ALP
350€
Synthesized peptide derived from human PCNA.
Human PCNA
Rabbit Anti-Human PCNA Polyclonal
Polyclonal Antibodies
ATLD2 Antibody, cb16 Antibody, Cyclin Antibody, DNA polymerase delta auxiliary protein Antibody, etID36690.10 Antibody, fa28e03 Antibody, fb36g03 Antibody, HGCN8729 Antibody, MGC8367 Antibody, Mutagen-sensitive 209 protein Antibody, OTTHUMP00000030189 Antibody, OTTHUMP00000030190 Antibody, PCNA Antibody, Pcna/cyclin Antibody, PCNA_HUMAN Antibody, PCNAR Antibody, Polymerase delta accessory protein Antibody, Proliferating cell nuclear antigen Antibody, wu:fa28e03 Antibody, wu:fb36g03 Antibodya
WB, IHC, ELISA
Rabbit
Human, Mouse, Rat
WB (1:1000), IHC (1:100), ELISA (1:5000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~29kDa, endogenous levels of total PCNA protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1307 was sufficient for detection of PCNA in 10 µg of HepG2 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Nucleus
See included datasheet or contact our support service.
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. It is a 36 kDa molecule that is highly conserved between species. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.
1. Moldovan GL. et al. (2007) Cell 129 (4): 665–79. 2. Warbrick E. et al. (1998) BioEssays 20 (3): 195–9. 3. Gilljam KM. et al. (2009) The Journal of Cell Biology 186 (5): 645–54.
6/Sep/2016
NP_872590.1
5111
P12004
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Immunohistochemistry analysis using Rabbit Anti-PCNA Polyclonal Antibody (SPC-1307). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (SPC-1307) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human HepG2 cell lysates showing detection of ~29kDa PCNA protein using Rabbit Anti-PCNA Polyclonal Antibody (SPC-1307). Lane 1: Human HepG2 cells. Lane 2: Human HepG2 cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (SPC-1307) at 1:1000. Predicted/Observed Size: ~29kDa. Rabbit Anti-PCNA Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1307) | Rabbit Anti-PCNA Antibody used in Western blot (WB) on HepG2 cell lysates (SPC-1307)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
