Immunogen
A synthesized peptide derived from human P Glycoprotein 1（MDR1）
Antibody's target
Human P Glycoprotein
Antibody's full description
Rabbit Anti-Human P Glycoprotein Polyclonal
100 µl
SPC-1306D-ALP
350€
A synthesized peptide derived from human P Glycoprotein 1（MDR1）
Human P Glycoprotein
Rabbit Anti-Human P Glycoprotein Polyclonal
Polyclonal Antibodies
ABC20 Antibody, ABCB1 Antibody, ATP binding cassette, sub family B (MDR/TAP), member 1 Antibody, ATP-binding cassette sub-family B member 1 Antibody, CD243 Antibody, CLCS Antibody, Colchicin sensitivity Antibody, Doxorubicin resistance Antibody, GP170 Antibody, MDR1 Antibody, MDR1_HUMAN Antibody, Multidrug resistance 1 Antibody, Multidrug resistance protein 1 Antibody, P glycoprotein 1 Antibody, P gp Antibody, P-glycoprotein 1 Antibody, PGY1 Antibody
IHC
Rabbit
Human, Mouse, Rat
WB (1:1000); IHC (1:200); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~141kDa, endogenous levels of total P Glycoprotein 1.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1306 was sufficient for detection of P Glycoprotein in 10 µg of PANC-1 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cell Membrane
Expressed in liver, kidney, small intestine and brain.
P-glycoprotein 1 (permeability glycoprotein, abbreviated as P-gp or Pgp) also known as multidrug resistance protein 1 (MDR1) or ATP-binding cassette sub-family B member 1 (ABCB1) or cluster of differentiation 243 (CD243) is an important protein of the cell membrane that pumps many foreign substances out of cells. The protein belongs to the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. P-gp is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood–brain barrier.
1. Aller SG et al. (2009) Science 323 (5922): 1718–22. 2. Ueda K et al. (1987) The Journal of Biological Chemistry 262 (2): 505–8. 3. Ferreira RJ et al. (2012) Journal of Chemical Theory and Computation 8 (6): 1853–64.
6/Sep/2016
NP_000918.2
5243
P08183
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human PANC-1 cell lysates showing detection of ~141kDa P Glycoprotein protein using Rabbit Anti-P Glycoprotein Polyclonal Antibody (SPC-1306). Lane 1: Human PANC-1 cells treated with the immunizing peptide. Lane 2: Human PANC-1 cells. Primary Antibody: Rabbit Anti-P Glycoprotein Polyclonal Antibody (SPC-1306) at 1:1000. Predicted/Observed Size: ~141kDa. Rabbit Anti-P Glycoprotein Antibody used in Western blot (WB) on PANC-1 cell lysates (SPC-1306)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus