Immunogen
Synthesized peptide derived from human osteopontin .
Antibody's target
Human Osteopontin
Antibody's full description
Rabbit Anti-Human Osteopontin Polyclonal
100 µl
SPC-1305D-ALP
350€
Synthesized peptide derived from human osteopontin .
Human Osteopontin
Rabbit Anti-Human Osteopontin Polyclonal
Polyclonal Antibodies
BNSP Antibody, Bone sialoprotein 1 Antibody, BSP I Antibody, BSPI Antibody, Early T lymphocyte activation 1 Antibody, ETA 1 Antibody, ETA1 Antibody, MGC110940 Antibody, Nephropontin Antibody, OPN Antibody, Osteopontin Antibody, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein Antibody, OSTP_HUMAN Antibody, PSEC0156 Antibody, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) Antibody, Secreted phosphoprotein 1 Antibody, SPP 1 Antibody, SPP-1 Antibody, SPP1 Antibody, SPP1/CALPHA1 fusion Antibody, Urinary stone protein Antibody, Uropontin Antibody
WB, ELISA
Rabbit
Human, Mouse, Rat
WB (1:1000), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~35kDa, endogenous levels of total osteopontin protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1305 was sufficient for detection of Osteopontin in 10 µg of LOVO cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Secreted
Bone. Found in plasma.
Osteopontin is a single-chain polypeptide. The gene has 7 exons, spans 5 kilobases in length and in humans it is located on the long arm of chromosome 4 region 22 (4q1322.1). The protein is composed of ~300 amino acids residues and has ~30 carbohydrate residues attached including 10 sialic acid residues, which are attached to the protein during post-translational modification in the Golgi apparatus. The protein is rich in acidic residues: 30-36% are either aspartic or glutamic acid. It is highly phosphorylated sialoprotein that is a prominent component of the mineralized extracellular matrices of bones and teeth. It is characterized by the presence of a polyaspartic acid sequence and sites of Ser/Thr phosphorylation that mediate hydroxyapatite binding, and a highly conserved RGD motif that mediates cell attachment/signaling.
1. Wang KX and Denhardt DT (2008) Cytokine Growth Factor Rev. 19 (5-6): 333–45. 2. Rangaswami H. et al. (2006) Trends Cell Biol. 16 (2): 79–87. 3. Young MF. et al. (1990) Genomics. 7 (1): 491–502.
6/Sep/2016
NP_000573.1
6696
P10451
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human LOVO cell lysates showing detection of ~35kDa Osteopontin protein using Rabbit Anti-Osteopontin Polyclonal Antibody (SPC-1305). Lane 1: Human LOVO cells. Lane 2: Human LOVO cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Osteopontin Polyclonal Antibody (SPC-1305) at 1:1000. Predicted/Observed Size: ~35kDa. Other Band(s): ~60kDa. Rabbit Anti-Osteopontin Antibody used in Western blot (WB) on LOVO cell lysates (SPC-1305)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus