Immunogen
A synthesized peptide derived from human NF-kappaB p105/p50
Antibody's target
Human NFKB1
Antibody's full description
Rabbit Anti-Human NFKB1 Polyclonal
100 µl
SPC-1303D-ALP
350€
A synthesized peptide derived from human NF-kappaB p105/p50
Human NFKB1
Rabbit Anti-Human NFKB1 Polyclonal
Polyclonal Antibodies
Embryonic stem cell specific homeobox protein (Nanog) Antibody, ENK Antibody, FLJ12581 Antibody, hNanog Antibody, Homeobox protein NANOG Antibody, Homeobox transcription factor Nanog Antibody, homeobox transcription factor Nanog-delta 48 Antibody, NANOG Antibody, Nanog homeobox Antibody, NANOG_HUMAN Antibody
WB, IHC, ICC/IF
Rabbit
Human, Mouse
WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~50kDa, endogenous levels of total NF-kappaB p105/p50
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1303 was sufficient for detection of NFKB1 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Nucleus, Cytoplasm
See included datasheet or contact our support service.
Nuclear factor NF-kappa-B p105 subunit is a protein that in humans is encoded by the NFKB1 gene. Nuclear factor kappa beta (NFKB) is a transcription factor that is highly influenced by stress, free-radicals, UV light and hypoxia. Unsurprisingly, NFKB is essential for many human cancers, as it regulates the expression of many downstream proteins that regulate or modify cellular growth and development. NFKB effects on angiogenic pathways and reactive mechanisms to stress and damage are well established throughout literature. Atherosclerosis is partly attributed to the mis-regulation of NFKB in hypoxia. Arthritis is related to inflammation proteins regulated through NFKB. It also has been shown to be important in synapses in brain, leading to memory and cognitive ability or impairment. In all, NFKB is one of the fastest responding transcription factors in humans, and is likely to mediate many responsive events in diseases and injury recovery.
1. Meyer R. et al. (1991) Proc Natl Acad Sci U S A 88 (3): 966–70. 2. Gerdin AK et al. (2010) Acta Ophthalmologica 88: 925–7.
6/Sep/2016
NP_003989.2
4790
P19838
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HeLa cell lysates showing detection of ~105kDa NFKB1 protein using Rabbit Anti-NFKB1 Polyclonal Antibody (SPC-1303). Lane 1: TNF-a treated Human HeLa whole cell lysates treated with the immunizing peptide. Lane 2: TNF-a treated Human HeLa whole cell lysate. Primary Antibody: Rabbit Anti-NFKB1 Polyclonal Antibody (SPC-1303) at 1:1000. Predicted/Observed Size: ~105kDa. Rabbit Anti-NFKB1 Antibody used in Western blot (WB) on HeLa cell lysates (SPC-1303)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus