Immunogen
A synthesized peptide derived from human Nanog
Antibody's target
Human Nanog
Antibody's full description
Rabbit Anti-Human Nanog Polyclonal
100 µl
SPC-1302D-ALP
350€
A synthesized peptide derived from human Nanog
Human Nanog
Rabbit Anti-Human Nanog Polyclonal
Polyclonal Antibodies
Embryonic stem cell specific homeobox protein (Nanog) Antibody, ENK Antibody, FLJ12581 Antibody, hNanog Antibody, Homeobox protein NANOG Antibody, Homeobox transcription factor Nanog Antibody, homeobox transcription factor Nanog-delta 48 Antibody, NANOG Antibody, Nanog homeobox Antibody, NANOG_HUMAN Antibody
WB, IHC, ICC/IF
Rabbit
Human, Mouse, Rat
WB (1:1000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~34kDa, endogenous levels of total Nanog.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1302 was sufficient for detection of Nanog in 10 µg of mouse tetis by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Nucleus
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes
NANOG is a transcription factor critically involved with self-renewal of undifferentiated embryonic stem cells. In humans, this protein is encoded by the NANOG gene. In stem cell development, the influence of the transcription factor Nanog is important for appropriate differentiation, or conversely to maintain their pluripotent state. Islet survivor cells, generated for progenitor cell testing, have nuclear expression of OCT4, SOX2 and Nanog, indicating a probability that they can maintain pluripotent characteristics in experimentation. In brain cancer, there are some stem cell like behaviors, and the cancer cells exhibiting these characteristics demonstrate high concentrations of Nanog, influenced by p53. Nanog seems to be very important for stem cell growth, maintenance and differentiation, but is usually co-expressed and co-activated by other factors, indicating the need for further mechanistic exploration into its activity.
1. Mitsui K. et al. (2003) Cell 113 (5): 631–42. 2. Chambers I. et al. (2003) Cell 113 (5): 643–55.
6/Sep/2016
NP_079141.2
79923
Q9H9S0
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus