Immunogen
A synthesized peptide derived from human MAP 2
Antibody's target
Human MAP2
Antibody's full description
Rabbit Anti-Human MAP2 Polyclonal
100 µl
SPC-1299D-ALP
350€
A synthesized peptide derived from human MAP 2
Human MAP2
Rabbit Anti-Human MAP2 Polyclonal
Polyclonal Antibodies
DKFZp686I2148 Antibody, MAP 2 Antibody, MAP dendrite specific Antibody, MAP-2 Antibody, MAP2 Antibody, MAP2A Antibody, MAP2B Antibody, MAP2C Antibody, Microtubule associated protein 2 Antibody, Microtubule-associated protein 2 Antibody, MTAP2_HUMAN Antibody
WB, IHC
Rabbit
Human, Mouse, Rat
WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~70kDa, endogenous levels of total MAP 2
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1299 was sufficient for detection of MAP2 in 10 µg of Brain cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cytoplasm, cytoskeleton
See included datasheet or contact our support service.
Microtubule-associated protein 2 (MAP2) is a protein that in humans is encoded by the MAP2 gene. MAP2 is essential for the development and maitenance of neuronal morphology. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neuritogenesis. MAP2 serves to stabilize microtubules (MT) growth by crosslinking MT with intermediate filaments and other MTs.
1. Neve R.L. et al. (1987) Brain Res 387 (3): 271–80. 2. Kalcheva N. et al. (1995) Proc Natl Acad Sci U S A 92 (24): 10894–8.
6/Sep/2016
NP_002365.3
4133
P11137
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Mouse Brain cell lysates showing detection of ~199kDa MAP2 protein using Rabbit Anti-MAP2 Polyclonal Antibody (SPC-1299). Lane 1: Mouse Brain cell lysate treated with the immunizing peptide. Lane 2: Mouse Brain cell lysate. Primary Antibody: Rabbit Anti-MAP2 Polyclonal Antibody (SPC-1299) at 1:1000. Predicted/Observed Size: ~199kDa. Rabbit Anti-MAP2 Antibody used in Western blot (WB) on Brain cell lysates (SPC-1299)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus