Immunogen
A synthesized peptide derived from human CXCR4
Antibody's target
Human CXCR4
Antibody's full description
Rabbit Anti-Human CXCR4 Polyclonal
100 µl
SPC-1293D-ALP
350€
A synthesized peptide derived from human CXCR4
Human CXCR4
Rabbit Anti-Human CXCR4 Polyclonal
Cancer, Neuroscience
Polyclonal Antibodies
C-X-C chemokine receptor type 4 Antibody, CD184 Antibody, CD184 antigen Antibody, Chemokine (C X C motif) receptor 4 Antibody, Chemokine CXC Motif Receptor 4 Antibody, CXC-R4 Antibody, CXCR-4 Antibody, CXCR4 Antibody, CXCR4_HUMAN Antibody, D2S201E Antibody, FB22 Antibody, Fusin Antibody, HM89 Antibody, HSY3RR Antibody, LAP 3 Antibody, LAP3 Antibody, LCR1 Antibody, LESTR Antibody, Leukocyte derived seven transmembrane domain receptor Antibody, Leukocyte-derived seven transmembrane domain receptor Antibody, Lipopolysaccharide associated protein 3 Antibody, Neuropeptide Y receptor Y3 Antibody, NPY3R Antibody, NPYR Antibody, NPYRL Antibody, NPYY3 Antibody, NPYY3R Antibody, Probable G protein coupled receptor lcr1 homolog Antibody, SDF 1 receptor Antibody, SDF-1 receptor Antibody, SEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR Antibody, Stromal cell derived factor 1 receptor Antibody, Stromal cell-derived factor 1 receptor Antibody, WHIM Antibody
WB, IHC
Rabbit
Human, Mouse, Rat
WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~45kDa, endogenous levels of total CXCR4.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1293 was sufficient for detection of CXCR4 in 10 µg of K562 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cell Membrane, Cell Junction, Early Endosome, Late Endosome, Lysosome
Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.
C-X-C chemokine receptor type 4 (CXCR-4) is a protein that in humans is encoded by the CXCR4 gene, also known as fusin or CD184 (cluster of differentiation 184). Chemokines and their receptors direct migration of distinct leukocyte subsets to sites of inflammation and to their specific niches in lymphoid organs. Virtually all T cell chemoattractants selectively attract memory/activated T cells. C-X-C chemokine receptor type 4 is involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. It has been associated with WHIM syndrome. WHIM like mutations in CXCR4 were recently identified in patients with Waldenstrom's macroglobulinemia, a B-cell malignancy.
1. Moriuchi M. et al. (1997) Journal of Immunology 159 (9): 4322–9. 2. Caruz A. et al. (1998) FEBS Letters 426 (2): 271–8. 3. Balabanian K. et al. (2008) The Journal of Clinical Investigation 118 (3): 1074–84. 4. Hunter Z.R. et al. (2014) Blood 123 (11): 1637–46.
6/Sep/2016
NP_003458.1
7852
P61073
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human K562 cell lysates showing detection of ~39kDa CXCR4 protein using Rabbit Anti-CXCR4 Polyclonal Antibody (SPC-1293). Lane 1: Human K562 cell lysate treated with the immunizing peptide. Lane 2: Human K562 cell lysate. Primary Antibody: Rabbit Anti-CXCR4 Polyclonal Antibody (SPC-1293) at 1:1000. Predicted/Observed Size: ~39kDa. Rabbit Anti-CXCR4 Antibody used in Western blot (WB) on K562 cell lysates (SPC-1293)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
