Immunogen
Synthetic peptide corresponding to a region derived human vascular endothelial growth factor A
Antibody's target
Human VEGF
Antibody's full description
Rabbit Anti-Human VEGF Polyclonal
100 µl
SPC-1291D-ALP
350€
Synthetic peptide corresponding to a region derived human vascular endothelial growth factor A
Human VEGF
Rabbit Anti-Human VEGF Polyclonal
Cardiovascular System, Angiogenesis, Growth Factors, VEGF, Cancer, Invasion/microenvironment, Cell Signaling, Metabolism, Metabolism processes, Hypoxia
Polyclonal Antibodies
Vascular Endothelial Growth Factor Antibody, Vascular Permeability Factor Antibody, VEGF-A Antibody, VEGFA Antibody, VEGFA_HUMAN Antibody, VPF Antibody
WB, IHC
Rabbit
Human, Mouse, Rat
WB (1:1000), IHC (1:200); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~24kDa and ~45kDa, endogenous level of total VEGFA protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1291 was sufficient for detection of VEGF in 10 µg of A431 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Secreted
Widely expressed (depending on isoform)
Vascular endothelial growth factor (VEGF), is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. VEGF stimulates cellular responses by binding to tyrosine kinase receptors on the cell surface. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF.
6/Sep/2016
NP_003367.4
7422
P15692
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human A431 cell lysates showing detection of ~28kDa VEGF protein using Rabbit Anti-VEGF Polyclonal Antibody (SPC-1291). Lane 1: Human A431 cell lysate treated with the immunizing peptide. Lane 2: Human A431 cell lysate. Primary Antibody: Rabbit Anti-VEGF Polyclonal Antibody (SPC-1291) at 1:1000. Predicted/Observed Size: ~28kDa. Rabbit Anti-VEGF Antibody used in Western blot (WB) on A431 cell lysates (SPC-1291)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
