Immunogen
A synthesized peptide derived from human VE-Cadherin
Antibody's target
Human VE Cadherin
Antibody's full description
Rabbit Anti-Human VE Cadherin Polyclonal
100 µl
SPC-1290D-ALP
350€
A synthesized peptide derived from human VE-Cadherin
Human VE Cadherin
Rabbit Anti-Human VE Cadherin Polyclonal
Cell Signaling, Cytoskeleton, Cell adhesion, Cancer, Invasion/microenvironment, Cardiovascular System, Cardiovascular Markers, Endothelial Cells
Polyclonal Antibodies
7B4 Antibody, Cadherin 5 Antibody, Cadherin 5 type 2 Antibody, cadherin, vascular endothelial Antibody, CD 144 Antibody, CDH 5 Antibody, Endothelial specific cadherin Antibody, Vascular endothelial cadherin Antibody, VE Cad Antibody
WB
Rabbit
Human, Mouse
WB (1:2000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of total VE-Cadherin
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1290 was sufficient for detection of VE Cadherin in 10 µg of Jurkat cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cell Junction, Cell membrane
Endothelial tissues and brain.
Cadherins are a super family of transmembrane glycoproteins that contain cadherin repeats of ~ 100 residues in their extracellular domain. They mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. Specifically, VE Cadherin plays an inportant role in endothelial cell biology. It associates with alpha-catenin forming a link to the cytoskeleton. And its signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis.
1. Wheelock M.J. and Johnson K.R. (2003) Annu Rev Cell Dev Biol. 19: 207-235. 2. Rabascio C., et al. (2004) Cancer Res. 64: 4373-4377. 3. Yamaoka-Tojo M., et al. (2006) Arterioscler Thromb Vasc Biol. 26: 1991-1997.
6/Sep/2016
NP_001786.2
1003
P33151
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human Jurkat cell lysates showing detection of ~120kDa VE Cadherin protein using Rabbit Anti-VE Cadherin Polyclonal Antibody (SPC-1290). Lane 1: Human Jurkat Whole Cell lysates treated with the immunizing peptide. Lane 2: Human Jurkat Whole Cell lysates. Primary Antibody: Rabbit Anti-VE Cadherin Polyclonal Antibody (SPC-1290) at 1:1000. Predicted/Observed Size: ~120kDa. Rabbit Anti-VE Cadherin Antibody used in Western blot (WB) on Jurkat cell lysates (SPC-1290)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
