Immunogen
Synthesized peptide derived from internal of human TIMP1.
Antibody's target
Human TIMP1
Antibody's full description
Rabbit Anti-Human TIMP1 Polyclonal
100 µl
SPC-1289D-ALP
350€
Synthesized peptide derived from internal of human TIMP1.
Human TIMP1
Rabbit Anti-Human TIMP1 Polyclonal
Cell Signaling, Extracellular Matrix (ECM), Cardiovascular System, Angiogenesis, Growth Factors, Adhesion / Extracellular Matrix, Matrix Metalloproteinases, Cancer, Invasion/microenvironment
Polyclonal Antibodies
Clgi Antibody, Collagenase inhibitor Antibody, Erythroid, Fibroblast collagenase inhibitor Antibody, HCI Antibody, Human Collagenase Inhibitor Antibody, Metalloproteinase inhibitor 1 Antibody, TIMP 1 Antibody, Tissue Inhibitor of Metalloproteinase 1 Antibody
WB, ELISA
Rabbit
Human, Mouse, Rat
WB (1:1000), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~23kDa, endogenous levels of total TIMP1 protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1289 was sufficient for detection of TIMP1 in 10 µg of HEK293 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Secreted
Detected in rheumatoid synovial fluid (at protein level).
Tissue inhibitors of metalloproteinases, or TIMPs are members of the family of tissue inhibitors of matrix metalloproteinases (MMPs). They exhibit multiple functions, including the inhibitory effect on MMPs, but they also they irreversibly inactivate MMPs by direct binding to their catalytic zinc cofactor and resultant inhibition of proteinase function. In addition they interact with various membrane receptors on the cell surface. TIMP1 in particular interacts with CD63. TIMPs are involved in a wide variety of biological functions, such as tumor angiogenesis and progression, wound healing, and vascular remodeling.
1. Nagase H., et al. (2006) Cardiovasc Res. 69: 562-573. 2. Visse R. and Nagase H. (2003) Circ Res. 92: 827-839. 3. Stetler-Stevenson W.G. (2008) Sci Signal. 1: re6. 4. Gill S.E. and Parks W.C. (2008) Int J Biochem Cell Biol. 40: 1334-1347. 5. Raffetto J.D. and Khalil R.A. (2008) Biochem Pharmacol. 75: 346-359.
6/Sep/2016
NP_003245.1
7076
P01033
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HEK293 cell lysates showing detection of ~25kDa TIMP1 protein using Rabbit Anti-TIMP1 Polyclonal Antibody (SPC-1289). Lane 1: Human HEK293 cells. Lane 2: HEK293 cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-TIMP1 Polyclonal Antibody (SPC-1289) at 1:1000. Predicted/Observed Size: ~25kDa. Rabbit Anti-TIMP1 Antibody used in Western blot (WB) on HEK293 cell lysates (SPC-1289)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
