Immunogen
A synthesized peptide derived from human SP1
Antibody's target
Human SP1
Antibody's full description
Rabbit Anti-Human SP1 Polyclonal
100 µl
SPC-1288D-ALP
350€
A synthesized peptide derived from human SP1
Human SP1
Rabbit Anti-Human SP1 Polyclonal
Stem Cells, Signaling Pathways, TGF Beta, Nuclear, Cell Signaling, Metabolism, Nucleotide Metabolism
Polyclonal Antibodies
TSFP1 Antibody, Transcription factor SP1 Antibody, SP 1 Antibody, Specificity protein 1 Antibody
WB, IHC, ICC/IF
Rabbit
Human, Mouse, Rat
WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~81kDa, endogenous levels of total SP1
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1288 was sufficient for detection of SP1 in 10 µg of HT29 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Nucleus, Cytoplasm
Up-regulated in adenocarcinomas of the stomach (at protein level).
SP1 is a ubiquitously expressed transcription factor that belongs to the SP/KLF family. It is 785 amino acids long, with a molecular weight of 81 kDA. It interacts with many other transciption factors such as c-Myc, EGR1 and Stat1, and has also been used as a control protein to compare with when studying the increase or decrease of the aryl hyrdocarbon receptor and/or the estrogen receptor.Activity and stability are regulated by post-translational modifications. SP1 has been found to be high in patients with Alzheimer's diease.
1. Tan N.Y. and Khachigian L.M. (2009) Mol Cell Biol. 29: 2483-2488. 2. Citron B.A. et al. (2008) J Neurosci Res 86, 2499-504. 3. Wormke M., et al. (2003) Mol. Cell. Biol. 23(6): 1843–1855.
6/Sep/2016
NP_612482.2
6667
P08047
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HT29 cell lysates showing detection of ~81kDa SP1 protein using Rabbit Anti-SP1 Polyclonal Antibody (SPC-1288). Lane 1: Human HT29 Whole Cell lysates treated with the immunizing peptide. Lane 2: Human HT29 Whole Cell lysates. Primary Antibody: Rabbit Anti-SP1 Polyclonal Antibody (SPC-1288) at 1:1000. Predicted/Observed Size: ~81kDa. Rabbit Anti-SP1 Antibody used in Western blot (WB) on HT29 cell lysates (SPC-1288)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
